Abstract Elephant grass ensilage might result in high dry matter (DM) loss and a reduction in feed quality. Knowledge of microbiota and the use of specific microbial inoculants can reduce DM losses and result in better fermentations and final products. The aim of the study was to characterise lactic acid bacteria (LAB) from elephant grass silages and subsequently select the promising strains to be used as microbial inoculants. Eighty-six LAB strains isolated from silages (7 from corn, 14 from sugarcane and 65 from elephant grass silages) were characterised and evaluated. Thirteen strains that showed the highest growth, pH reduction, production of interest metabolites and the ability to inhibit undesirable microorganisms and the strain L. hilgardii CCMA 0170 were evaluated in experimental silos. The aerobic stability, fermentative profile and microbial population of elephant grass silages of BRS Capiacu cultivar were evaluated after 60 days of storage. No LAB was found in elephant grass before ensiling. The elephant grass silage fermentation was characterised by the succession of Lactobacillus sakei, L. plantarum group, Pediococcus pentosaceus and L. brevis. The silages inoculated with CCMA 0170 (L. hilgardii) CCMA 1394 and CCMA 1402 (L. plantarum), CCMA 1409 (L. brevis), CCMA 1363 and CCMA 1364 (L. farraginis) showed the smallest DM loss (mean of 38 g/kg of ensiled vs. 123 g/kg in control silage) and high aerobic stability (mean of 33.1 h vs. 21.2 h in control silage). Among the tested strains, the CCMA 1364 strain stands out as resulting in silages with low yeast populations, undetectable levels of butyric acid and high aerobic stability. Our results highlight the importance of the research and evaluation of new strains adapted to the fermentative process of each forage.

中文翻译：

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新型乳酸菌菌株增强了象草青贮 cv 的保护。BRS卡皮亚苏

摘要 象草青贮可能会导致高干物质 (DM) 损失和饲料质量下降。微生物群的知识和特定微生物接种剂的使用可以减少 DM 损失并导致更好的发酵和最终产品。该研究的目的是表征来自大象草青贮饲料的乳酸菌 (LAB)，然后选择有希望用作微生物接种剂的菌株。从青贮饲料中分离出的 86 个 LAB 菌株（7 个来自玉米，14 个来自甘蔗，65 个来自象草青贮）被表征和评估。在实验筒仓中评估了 13 种显示出最高生长、pH 值降低、产生感兴趣的代谢物和抑制不良微生物的能力的菌株和菌株 L. hilgardii CCMA 0170。有氧稳定性，在储存 60 天后评估了 BRS Capiacu 品种的象草青贮饲料的发酵特征和微生物种群。青贮前在象草中未发现乳酸菌。象草青贮发酵具有清酒乳杆菌、植物乳杆菌群、戊糖片球菌和短乳杆菌的演替特征。接种 CCMA 0170 (L. hilgardii) CCMA 1394 和 CCMA 1402 (L. plantarum)、CCMA 1409 (L. brevis)、CCMA 1363 和 CCMA 1364 (L. farraginis) 的青贮饲料的 DM 损失最小（平均 38 g /kg 青贮与对照青贮饲料中的 123 g/kg）和高需氧稳定性（平均 33.1 小时与对照青贮饲料中的 21.2 小时）。在测试的菌株中，CCMA 1364 菌株脱颖而出，因为青贮饲料的酵母菌数量少、丁酸水平不可检测且有氧稳定性高。