The present study was designed to identify nine Arcanobacterium phocae strains isolated from cases of mink dermatitis of a single farm in Finland and characterize the strains for epidemiological relationships. All nine strains and previously described A. phocae used for comparative purposes were identified and further characterized phenotypically, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of phocaelysin encoding gene phl with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing 16S rRNA gene and gene phl, the elongation factor tu encoding gene tuf and the β subunit of bacterial RNA polymerase encoding gene rpoB. Genetic relatedness among isolates was determined using whole-genome single nucleotide polymorphism (wgSNP) analysis. The wgSNP results, partly the MALDI-TOF MS and FT-IR analyses and sequencing of the genes, revealed that the nine A. phocae strains recovered from a single farm showed close sequence similarities among each other and differed from previously investigated A. phocae strains isolated from other farms and animals in Finland and from the A. phocae type strain. This indicated a close epidemiological relationship of the A. phocae strains isolated from a single farm and that the nine A. phocae strains of the present study might have developed from a common ancestor.

本研究旨在鉴定从芬兰单个农场的水貂皮炎病例中分离出的9株Arcanobacterium phocae菌株，并确定这些菌株与流行病学之间的关系。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS），傅里叶变换红外光谱（FT-IR），鉴定并比较了用于比较目的的所有9个菌株和先前描述的A.phocae，并在表型上进行了表征。和基因型上，通过用先前开发的环介导的等温扩增（LAMP）分析检测磷脂酰肌醇溶蛋白编码基因phl，并通过对16S rRNA基因和基因phl测序，延伸因子tu编码基因tuf和细菌RNA聚合酶编码基因rpoB的β亚基。使用全基因组单核苷酸多态性（wgSNP）分析确定了分离株之间的遗传相关性。wgSNP结果（部分通过MALDI-TOF MS和FT-IR分析以及对基因的测序）显示，从单个农场中回收的9个磷藻菌株彼此之间具有相似的序列相似性，并且与以前调查的磷藻菌株不同分离自芬兰其他农场和动物以及A.phocae型菌株。这表明从单个农场分离出的A. phocae菌株与九种A. phocae密切的流行病学关系 本研究的菌株可能是从一个共同的祖先发育而来的。