This work aimed at characterizing four Staphylococcus aureus and 68 coagulase-negative staphylococci (CoNS), recovered from the air and liquid manure tank of two swine farms with intensive- and semi-extensive-production types, for their antimicrobial resistance pheno-/genotypes and their virulence gene content. Molecular typing was performed by spa typing, MLST, agr typing, and SCCmec typing, where applicable. Conjugation experiments were performed to assess the transferability of the linezolid resistance gene cfr, and its genetic environment was determined by Whole-Genome-Sequencing. The four S. aureus (intensive-production farm, IP-farm) were typed as t011-agrI-CC398-ST398, were scn-negative and two of them were methicillin-resistant (MRSA) with the mecA gene (SCCmec-V). Multidrug resistance was seen in 87 % of the CoNS. Statistically significant differences among the antimicrobial resistance rates of CoNS from the two farms were observed for cefoxitin, aminoglycosides, tetracycline, ciprofloxacin and trimethoprim-sulfamethoxazole. Eight methicillin-resistant CoNS, which were recovered from the IP-farm, carried the mecA gene. One S. simulans isolate was PVL-positive and three S. cohnii eta-positive. One S. equorum and one S. arlettae showed linezolid resistance and carried the cfr gene (IP-farm), which was non-transferable by conjugation into S. aureus. The cfr genetic context in both isolates was identical, with the lsa(B) gene located upstream of cfr. The environment of swine farms might contribute to the dissemination of CoNS that show multidrug resistance and harbor important virulence factors.

这项工作旨在鉴定从两个集约化和半集约化生产型猪场的空气和液体肥料罐中回收的四种金黄色葡萄球菌和68种凝固酶阴性葡萄球菌（CoNS）的抗菌素耐药表型/基因型和它们的毒力基因含量。分子分型通过水疗分型，MLST，agr分型和SCC mec分型（如果适用）进行。进行共轭实验以评估利奈唑胺抗性基因cfr的可转移性，并通过全基因组测序确定其遗传环境。将四个金黄色葡萄球菌（集约化生产农场，IP农场）键入为t011- agrI-CC398-ST398是scn阴性，其中两个对带有mecA基因（SCC mec -V）的耐甲氧西林（MRSA ）。在87％的CoNS中发现了多药耐药性。对于头孢西丁，氨基糖苷类，四环素，环丙沙星和甲氧苄氨嘧啶-磺胺甲基异恶唑，两个农场的CoNS的耐药率在统计学上有显着差异。从IP农场回收的八个耐甲氧西林的CoNS带有mecA基因。一种S. simulans分离株为PVL阳性，而三株S. cohnii eta阳性。一个S. equorum和一个S. arlettae显示利奈唑胺抗性和所携带的cfr基因（IP-farm），无法与金黄色葡萄球菌结合而转移。两种分离物中的cfr遗传背景是相同的，而lsa（B）基因位于cfr的上游。养猪场的环境可能有助于CoNS的传播，这些CoNS表现出多药耐药性并具有重要的毒力因子。