Biological meshes have always posed a challenge in biological medicine, for which nanocomposites with enhanced biocompatibility and antibacterial activity may be beneficial. In this study, lysozyme (LY) and collagen (Col) were alternately deposited on silk fibroin (SF) and nylon 6 (N6) composite nanofibrous mats using a layer-by-layer (LBL) self-assembly technique. The mechanical properties, biocompatibility, and antibacterial activity of the LBL structured mats were characterized systematically to investigate the impact of the LBL process on the biological properties of SF/N6 nanofibrous mats. Our results showed that the effective deposition of LY and Col may affect the surface topography, mechanical properties, and wetting behavior of the SF/N6 nanofibrous mats. Moreover, LBL structured mats exhibited excellent biocompatibility and antibacterial properties. Among all the tested mats, those coated with 10 bilayers of LY and Col displayed the best biocompatibility, and relatively good mechanical and antibacterial properties. Thus, LBL structured mats, especially those with a 10 bilayer coating, are potentially valuable in clinical therapy for pelvic organ prolapse in the future.

生物网在生物医学中一直是一个挑战，具有增强的生物相容性和抗菌活性的纳米复合材料可能是有益的。在这项研究中，溶菌酶（LY）和胶原蛋白（Col）使用层层（LBL）自组装技术交替沉积在丝素蛋白（SF）和尼龙6（N6）复合纳米纤维垫上。系统地表征了LBL结构垫的机械性能，生物相容性和抗菌活性，以研究LBL工艺对SF / N6纳米纤维垫的生物学性能的影响。我们的结果表明，LY和Col的有效沉积可能会影响SF / N6纳米纤维垫的表面形貌，力学性能和润湿行为。此外，LBL结构垫具有出色的生物相容性和抗菌性能。在所有测试过的垫子中，涂有LY和Col双层10层的垫子表现出最佳的生物相容性，以及相对较好的机械和抗菌性能。因此，LBL结构的垫子，尤其是具有10个双层涂层的垫子，在将来用于盆腔器官脱垂的临床治疗中具有潜在的价值。