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Expression profiling of the Kdm genes in scallop Patinopecten yessoensis suggests involvement of histone demethylation in regulation of early development and gametogenesis.
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 2.2 ) Pub Date : 2020-03-19 , DOI: 10.1016/j.cbpb.2020.110434
Zhenyi Guo 1 , Lijing Zhang 1 , Yajuan Li 1 , Shaoxuan Wu 1 , Shi Wang 2 , Lingling Zhang 3 , Zhenmin Bao 3
Affiliation  

Histone demethylation modification is an important means of gene expression regulation and is widely involved in biological processes such as animal reproduction and development. Histone lysine demethylases (Kdm) plays an important role in the demethylation of histones. To understand the role of histone demethylation in scallops, we identified the Kdm gene family of the Yesso scallop Patinopecten yessoensis, and analyzed its expression during the gonad and early development. The results showed that the P. yessoensis has a complete Kdm family including seventeen members that belong to sixteen subfamilies (Hif1an, Hspbap1, Jarid2, Jmjd4, Jmjd6, Jmjd7, Jmjd8, Kdm1, Kdm2, Kdm3, Kdm4, Kdm5, Kdm6, Kdm7, Kdm8 and Kdm9). The Kdm genes showed five different expression patterns in the early development of scallop, with some of them (e.g. Jmjd7, Jmjd8 and Kdm8) being highly expressed in only one or two stage and the others (e.g. Kdm1A, Kdm9, Jmjd4 and Jmjd6) in several consecutive stages. During gonadal development, the Kdm genes also display various expression patterns. Some genes (e.g. Kdm2, Kdm4 and Jmjd7) display preferential expression in the testis, and the others have no obvious sex bias but show stage preference (resting, proliferative, growing or maturation stage). These results suggest that various histone demethylation modifications (e.g. H3K4, H3K9 and H3K27) may participate in the regulation of gametogenesis and early development of Yesso scallop. It will facilitate a better understanding of the epigenetic contributions to mollusk development.

中文翻译:

扇贝Patinopecten yessoensis中Kdm基因的表达谱表明组蛋白去甲基化参与早期发育和配子发生的调节。

组蛋白去甲基化修饰是基因表达调节的重要手段,并广泛参与诸如动物繁殖和发育的生物学过程。组蛋白赖氨酸脱甲基酶(Kdm)在组蛋白的脱甲基中起重要作用。为了了解组蛋白去甲基化在扇贝中的作用,我们鉴定了Yesso扇贝Patinopecten yessoensis的Kdm基因家族,并分析了其在性腺和早期发育中的表达。结果表明,耶氏疟原虫具有完整的Kdm家族,包括17个成员,属于16个亚家族(Hif1an,Hspbap1,Jarid2,Jmjd4,Jmjd6,Jmjd7,Jmjd8,Kdm1,Kdm2,Kdm3,Kdm4,Kdm5,Kdm5,Kdm5 Kdm8和Kdm9)。Kdm基因在扇贝的早期发育中表现出五种不同的表达模式,其中一些(例如Jmjd7,Jmjd8和Kdm8)仅在一个或两个阶段中得到高度表达,而其他阶段(例如Kdm1A,Kdm9,Jmjd4和Jmjd6)则在几个连续阶段中得到高度表达。在性腺发育过程中,Kdm基因还表现出多种表达模式。一些基因(例如Kdm2,Kdm4和Jmjd7)在睾丸中表现出优先表达,而其他基因则没有明显的性别偏见,但表现出阶段性偏好(静止,增殖,生长或成熟阶段)。这些结果表明,各种组蛋白去甲基化修饰(例如H3K4,H3K9和H3K27)可能参与配子的形成和扇贝的早期发育。这将有助于更好地了解表观遗传对软体动物发育的贡献。在性腺发育过程中,Kdm基因还表现出多种表达模式。一些基因(例如Kdm2,Kdm4和Jmjd7)在睾丸中表现出优先表达,而其他基因则没有明显的性别偏见,但表现出阶段性偏好(静止,增殖,生长或成熟阶段)。这些结果表明,各种组蛋白去甲基化修饰(例如H3K4,H3K9和H3K27)可能参与配子的形成和扇贝的早期发育。这将有助于更好地了解表观遗传对软体动物发育的贡献。在性腺发育过程中,Kdm基因还表现出多种表达模式。一些基因(例如Kdm2,Kdm4和Jmjd7)在睾丸中表现出优先表达,而其他基因则没有明显的性别偏见,但表现出阶段性偏好(静止,增殖,生长或成熟阶段)。这些结果表明,各种组蛋白去甲基化修饰(例如H3K4,H3K9和H3K27)可能参与配子的形成和扇贝的早期发育。这将有助于更好地了解表观遗传对软体动物发育的贡献。生长或成熟阶段)。这些结果表明,各种组蛋白去甲基化修饰(例如H3K4,H3K9和H3K27)可能参与配子的形成和扇贝的早期发育。这将有助于更好地了解表观遗传对软体动物发育的贡献。生长或成熟阶段)。这些结果表明,各种组蛋白去甲基化修饰(例如H3K4,H3K9和H3K27)可能参与配子的形成和扇贝的早期发育。这将有助于更好地了解表观遗传对软体动物发育的贡献。
更新日期:2020-03-20
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