Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening. The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords. The full chemical-defined medium supporting the isolation and expansion of human MSCs has been developed. This would be helpful for further optimization of the MSC medium, their clinical applications, and molecular characterization.

中文翻译：

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化学定义的培养基，支持人间充质干细胞的扩增。

间充质干细胞（MSCs）的治疗潜力已得到深入研究。但是，尚未开发出支持人类MSC分离和扩增的完全化学成分确定的培养基。在这里，我们通过RNA测序，生物信息学分析和生长因子筛选，开发了完全化学定义的培养基NBVbe培养基。NBVbe培养基包含N2B27培养基，其中BSA（牛血清白蛋白）被重组人白蛋白，bFGF（碱性成纤维细胞生长因子），维生素C和EGF（表皮生长因子）替代。NBVbe培养基可以支持从脐带中分离和扩增人MSC。已经开发出支持人MSC分离和扩增的完全化学成分确定的培养基。这将有助于进一步优化MSC介质，