Crop productivity is challenged by abiotic stresses, among which drought stress is the most common. NF-Y genes, especially NF-YA genes, regulate tolerance to abiotic stress. Soybean NF-Y gene GmNFYA5 was identified to have the highest transcript level among all 21 NF-YA genes in soybean (Glycine max L.) under drought stress. Drought-induced transcript of GmNFYA5 was suppressed by the ABA synthesis inhibitor naproxen (NAP). GmNFYA5 transcript was detected in various tissues at vegetative and reproductive growth stages with higher levels in roots and leaves than in other tissues, which was consist with the GmNFYA5 promoter: GUS fusion assay. Overexpression of GmNFYA5 in transgenic Arabidopsis plants caused enhanced drought tolerance in seedlings by decreasing stomatal aperture and water loss from leaves. Overexpression and suppression of GmNFYA5 in soybean resulted in increased and decreased drought tolerance, respectively, relative to plants with an empty vector (EV). Transcript levels of ABA-dependent genes (ABI2, ABI3, NCED3, LEA3, RD29A, P5CS1, GmWRKY46, GmNCED2 and GmbZIP1) and ABA-independent genes (DREB1A, DREB2A, DREB2B, GmDREB1, GmDREB2 and GmDREB3) in transgenic plants overexpressing GmNFYA5 were higher than those of wild-type plants under drought stress; suppression of GmNFYA5 transcript produced opposite results. GmNFYA5 probably regulated the transcript abundance of GmDREB2 and GmbZIP1 by binding to the promoters in vivo. Our results suggested that overexpression of GmNFYA5 improved drought tolerance in soybean via both ABA-dependent and ABA-independent pathways.

中文翻译：

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GmNFYA5的过表达赋予转基因拟南芥和大豆植物抗旱性

作物生产力受到非生物胁迫的挑战，其中最常见的是干旱胁迫。NF-Y基因，尤其是NF-YA基因，调节对非生物胁迫的耐受性。在干旱胁迫下，大豆（Glycine max L.）的所有21个NF-YA基因中，大豆NF-Y基因GmNFYA5的转录水平最高。干旱诱导的GmNFYA5转录本被ABA合成抑制剂萘普生（NAP）抑制。在营养和生殖生长阶段的各种组织中检测到GmNFYA5转录本，其根和叶中的水平高于其他组织，这由GmNFYA5启动子：GUS融合测定组成。GmNFYA5在转基因拟南芥植物中的过表达通过降低气孔孔径和叶片水分流失而增强了幼苗的耐旱性。相对于带有空载体（EV）的植物，大豆中GmNFYA5的过表达和抑制分别导致干旱耐受性的提高和降低。ABA依赖基因（ABI2，ABI3，NCED3，LEA3，RD29A，P5CS1，GmWRKY46，GmNCED2和GmbZIP1）和ABA非依赖基因（DREB1A，DREB2A，DREB2B，GmDREB1，GmDREBing2和GmDREB3的转录水平）处于转录水平高于干旱胁迫下的野生型植物；抑制GmNFYA5转录产生相反的结果。GmNFYA5可能通过与体内的启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。相对于带有空载体（EV）的植物。ABA依赖基因（ABI2，ABI3，NCED3，LEA3，RD29A，P5CS1，GmWRKY46，GmNCED2和GmbZIP1）和ABA非依赖基因（DREB1A，DREB2A，DREB2B，GmDREB1，GmDREB2和GmDREB3的转录水平）处于转录水平高于干旱胁迫下的野生型植物；抑制GmNFYA5转录产生相反的结果。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。相对于带有空载体（EV）的植物。ABA依赖基因（ABI2，ABI3，NCED3，LEA3，RD29A，P5CS1，GmWRKY46，GmNCED2和GmbZIP1）和ABA非依赖基因（DREB1A，DREB2A，DREB2B，GmDREB1，GmDREB2和GmDREB3的转录水平）处于转录水平高于干旱胁迫下的野生型植物；抑制GmNFYA5转录产生相反的结果。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。在干旱胁迫下，过表达GmNFYA5的转基因植物中的GmNCED2和GmbZIP1）和不依赖ABA的基因（DREB1A，DREB2A，DREB2B，GmDREB1，GmDREB2和GmDREB3）高于野生型植物。抑制GmNFYA5转录产生相反的结果。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。在干旱胁迫下，过表达GmNFYA5的转基因植物中的GmNCED2和GmbZIP1）和不依赖ABA的基因（DREB1A，DREB2A，DREB2B，GmDREB1，GmDREB2和GmDREB3）高于野生型植物。抑制GmNFYA5转录产生相反的结果。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。GmNFYA5可能通过在体内与启动子结合来调节GmDREB2和GmbZIP1的转录本丰度。我们的结果表明，GmNFYA5的过表达通过ABA依赖性和ABA依赖性途径改善了大豆的耐旱性。