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Antioxidant Enzyme Activity and Lipid Peroxidation in Aporrectodea caliginosa Earthworms Exposed to Silver Nanoparticles and Silver Nitrate in Spiked Soil.
Environmental Toxicology and Chemistry ( IF 4.1 ) Pub Date : 2020-03-18 , DOI: 10.1002/etc.4713
Nadir Saleeb 1 , Brett Robinson 1 , Jo Cavanagh 2 , James Ross 1 , Kiran Munir 1 , Ravi Gooneratne 1
Affiliation  

Silver nanoparticles (AgNPs) from industrial use, discharged via the land application of sewage sludge, are interacting with soil biota, including earthworms. In affected organisms, excessive production of reactive oxygen species can result in lipid peroxidation, shifting the balance between oxidants and antioxidants to cause oxidative stress. We determined selected lower-tier biomarkers such as antioxidant responses and lipid peroxidation in Aporrectodea caliginosa earthworms exposed to soils spiked with AgNPs or silver nitrate (AgNO3 ). Aporrectodea caliginosa were exposed to AgNPs at 0 (control), 0.3, 3, 30, and 300 mg/kg or Ag+ (as AgNO3 ) at 0, 0.03, 0.3, 3, and 10 mg/kg in soil for 4 wk. At 1, 2, 3, and 4 wk, the activity of the antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, as well as lipid peroxidation (malondialdehyde content), increased as a function of concentration, with a much larger response for Ag+ than AgNPs. Given the likelihood of ever-increasing AgNP concentrations in soil, where AgNPs can transform to ionic Ag (Ag+ ), our findings of antioxidant response to oxidative stress in a common indicator organism even at an environmentally realistic exposure concentration of 0.03 mg/kg demonstrate that AgNPs may affect soil fertility and, thus, agricultural production. Evaluating selected lower-tier biomarkers offers a meaningful assessment of AgNPs and Ag+ effects on terrestrial earthworms. Environ Toxicol Chem 2020;39:1257-1266. © 2020 SETAC.

中文翻译:

尖峰土壤中暴露于银纳米颗粒和硝酸银的A游孢线Earth的抗氧化酶活性和脂质过氧化作用

工业用途的银纳米颗粒(AgNPs)通过污水污泥在土地上的排放而排放,正在与土壤生物(包括earth)相互作用。在受影响的生物中,活性氧物质的过量产生会导致脂质过氧化,从而改变氧化剂和抗氧化剂之间的平衡,从而引起氧化应激。我们在暴露于掺有AgNPs或硝酸银(AgNO3)的土壤中的caliporosa caliporosa worm中确定了选定的较低层生物标志物,例如抗氧化剂响应和脂质过氧化作用。在土壤中以0(对照组),0.3、3、30和300 mg / kg的AgNPs或以0、0.03、0.3、3和10 mg / kg的Ag +(作为AgNO3)暴露于土壤中4周。在1、2、3和4周时,抗氧化酶超氧化物歧化酶,过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽S转移酶,脂质过氧化(丙二醛含量)随浓度增加而增加,对Ag +的响应要比对AgNP的响应大得多。考虑到土壤中AgNP浓度可能不断增加的趋势,其中AgNPs可以转化为离子Ag(Ag +),因此即使在环境中实际暴露浓度为0.03 mg / kg的情况下,我们在常见指示生物中对氧化应激的抗氧化反应的发现也表明: AgNPs可能会影响土壤肥力,从而影响农业生产。评估选定的较低层生物标记可对AgNPs和Ag +对陆生effects的作用提供有意义的评估。Environ Toxicol Chem 2020; 39:1257-1266。©2020 SETAC。考虑到土壤中AgNP浓度可能不断增加的趋势,其中AgNPs可以转化为离子Ag(Ag +),因此即使在环境中实际暴露浓度为0.03 mg / kg的情况下,我们在常见指示生物中对氧化应激的抗氧化反应的发现也表明: AgNPs可能会影响土壤肥力,从而影响农业生产。评估选定的较低层生物标记可对AgNPs和Ag +对陆生effects的作用提供有意义的评估。Environ Toxicol Chem 2020; 39:1257-1266。©2020 SETAC。考虑到土壤中AgNP浓度可能不断增加的趋势,其中AgNPs可以转化为离子Ag(Ag +),因此即使在环境中实际暴露浓度为0.03 mg / kg的情况下,我们在常见指示生物中对氧化应激的抗氧化反应的发现也表明: AgNPs可能会影响土壤肥力,从而影响农业生产。评估选定的较低层生物标记可对AgNPs和Ag +对陆生effects的作用提供有意义的评估。Environ Toxicol Chem 2020; 39:1257-1266。©2020 SETAC。农业生产。评估所选的较低层生物标记可对AgNPs和Ag +对陆地on的作用提供有意义的评估。Environ Toxicol Chem 2020; 39:1257-1266。©2020 SETAC。农业生产。评估选定的较低层生物标记可对AgNPs和Ag +对陆生effects的作用提供有意义的评估。Environ Toxicol Chem 2020; 39:1257-1266。©2020 SETAC。
更新日期:2020-03-18
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