The nicotinamide biomimetic BNAH has been demonstrated to serve as an effective reductant for generating reduced flavin in the chemoenzymatic cascade reaction of bacterial luciferase. This cascade reaction is less complex, has greater ease of use, and is more cost effective. The technology developed is useful as a tool for future applications of bacterial luciferase in high‐throughput detection assays for drug and natural compound screening.

中文翻译：

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用于生物报告基因应用的细菌萤光素酶的最小化化学级联反应。

烟酰胺仿生剂BNAH已被证明是一种有效的还原剂，可在细菌荧光素酶的化学酶联反应中产生减少的黄素。这种级联反应不那么复杂，具有更高的易用性，并且更具成本效益。所开发的技术可用作细菌荧光素酶在药物和天然化合物筛选的高通量检测分析中未来应用的工具。