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Optical Control of Cellular ATP Levels with a Photocaged Adenylate Kinase.
ChemBioChem ( IF 3.2 ) Pub Date : 2020-03-18 , DOI: 10.1002/cbic.201900757 Wenyuan Zhou 1 , Chasity P Hankinson 1 , Alexander Deiters 1
ChemBioChem ( IF 3.2 ) Pub Date : 2020-03-18 , DOI: 10.1002/cbic.201900757 Wenyuan Zhou 1 , Chasity P Hankinson 1 , Alexander Deiters 1
Affiliation
Direct optical perturbation of cellular ATP levels is achieved by substituting the essential lysine of E. coli adenylate kinase with a hydroxycoumarin‐protected lysine through unnatural amino acid mutagenesis. The caged lysine residue renders the kinase inactive until photolysis reactivates it in both E. coli and mammalian cells.
中文翻译:
光笼罩的腺苷酸激酶对细胞ATP水平的光学控制。
通过非天然氨基酸诱变,用羟基香豆素保护的赖氨酸取代大肠杆菌腺苷酸激酶的必需赖氨酸,可以实现细胞ATP水平的直接光学扰动。笼罩的赖氨酸残基使该激酶失活,直到在大肠杆菌和哺乳动物细胞中光解将其重新活化为止。
更新日期:2020-03-18
中文翻译:
光笼罩的腺苷酸激酶对细胞ATP水平的光学控制。
通过非天然氨基酸诱变,用羟基香豆素保护的赖氨酸取代大肠杆菌腺苷酸激酶的必需赖氨酸,可以实现细胞ATP水平的直接光学扰动。笼罩的赖氨酸残基使该激酶失活,直到在大肠杆菌和哺乳动物细胞中光解将其重新活化为止。