Abstract Biological sulfate reduction is an established method leading to new biotechnologies in wastewater treatment. Batch assays are a powerful tool to support sulfate-reducing bacteria (SRB) research and application; however, their methodology is far from been standardized. The present review critically analyses the experimental conditions used for SRB batch assays in literature, to propose a general experimental framework supporting a future standardized protocol. Batch assays using SRB biomass (SRB-test) were grouped in three different categories depending on their objective: (1) test assessing activity of a SRB culture, (2) test determining the reduction potential of an electron donor, and (3) test determining the feasibility of using different sulfate sources as electron acceptor. Considering the methodologies and results from the reviewed publications, set-up and monitoring conditions are recommended to enhance SRB-tests comparability and reproducibility. The recommendations include the use of lactate as electron donor and sodium sulfate as electron acceptor as controls in the SRB-tests. Finally, due to the difficulty to standardize the biomass source, the microbial characterization of the biomass is recommended.

中文翻译：

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生物硫酸盐还原的批量分析：对标准化方案的审查

摘要 生物硫酸盐还原是一种成熟的方法，可在废水处理中产生新的生物技术。批量分析是支持硫酸盐还原菌 (SRB) 研究和应用的有力工具；然而，他们的方法远未标准化。本综述批判性地分析了文献中用于 SRB 批量检测的实验条件，以提出支持未来标准化协议的通用实验框架。使用 SRB 生物量的批量分析（SRB 测试）根据其目的分为三个不同的类别：（1）测试评估 SRB 培养物的活性，（2）测试确定电子供体的还原电位，以及（3）测试确定使用不同硫酸盐源作为电子受体的可行性。考虑到已审查出版物的方法和结果，建议设置和监测条件以提高 SRB 测试的可比性和可重复性。建议包括在 SRB 测试中使用乳酸盐作为电子供体和硫酸钠作为电子受体作为对照。最后，由于难以标准化生物质来源，建议对生物质进行微生物表征。