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Arabidopsis KETCH1 Is Critical for the Nuclear Accumulation of Ribosomal Proteins and Gametogenesis
The Plant Cell ( IF 11.6 ) Pub Date : 2020-02-21
Xiong, F., Duan, C.-Y., Liu, H.-H., Wu, J.-H., Zhang, Z.-H., Li, S., Zhang, Y.

\Male and female gametophytes are generated from micro- or megaspore mother cells through consecutive meiotic and mitotic cell divisions, whose defects often result in gametophytic lethality. Gametophytic lethality was also reported when genes encoding ribosome-related proteins were mutated. Although a large amount of ribosomal proteins (RPs) have been identified in plants based on homology with their yeast and metazoan counterparts, how RPs are regulated, such as dynamic subcellular targeting, is little known. We report here that an Arabidopsis importin β, KETCH1 (karyopherin enabling the transport of the cytoplasmic HYL1), is critical for gametogenesis. Karyopherins are molecular chaperons mediating nucleocytoplasmic protein transport. However, the role of KETCH1 during gametogenesis is independent of HYPONASTIC LEAVES 1 (HYL1), a previously reported KETCH1 cargo. Instead, KETCH1 interacts with several RPs and is critical for the nuclear accumulation of RPL27a, whose mutations caused similar gametophytic defects. We further showed that knocking down KETCH1 caused reduced ribosome biogenesis and translational capacity, which may trigger the arrest of mitotic cell cycle progression and lead to gametophytic lethality.



中文翻译:

拟南芥KETCH1对于核糖体蛋白的核积累和配子发生至关重要

雄配子体和雌配子体是通过连续的减数分裂和有丝分裂细胞分裂从小孢子或大孢子母细胞产生的,它们的缺陷通常导致配子体的致死性。当编码核糖体相关蛋白的基因突变时,也报道了配子体致死性。尽管已经基于与它们的酵母和后生动物对应物的同源性在植物中鉴定出大量核糖体蛋白(RPs),但是鲜为人知如何调节RPs,例如动态亚细胞靶向。我们在这里报告,拟南芥importinβ,KETCH1(核转运蛋白使细胞质HYL1的运输),是配子发生的关键。核仁蛋白是介导核质蛋白转运的分子伴侣。但是,KETCH1在配子发生过程中的作用独立于半透明叶1(HYL1),先前报告的KETCH1货物。相反,KETCH1与多个RP相互作用,对于RPL27a的核积累至关重要,RPL27a的突变引起类似的配子缺陷。我们进一步表明,敲低KETCH1会导致核糖体生物发生和翻译能力降低,这可能会触发有丝分裂细胞周期进程的停止并导致配子体致死性。

更新日期:2020-03-19
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