The Arabidopsis cyclin-dependent kinase G1 (CDKG) is necessary for recombination and synapsis during male meiosis at high ambient temperature. In the cdkg1-1 mutant, synapsis is impaired and there is a dramatic reduction in the number of class I crossovers resulting in univalents at metaphase I and pollen sterility. Here we demonstrate that CDKG1 is necessary for the processing of recombination intermediates in the canonical ZMM recombination pathway and that loss of CDKG1 results in increased class II COs. While synapsis and events associated with class I crossovers are severely compromised in a cdkg1-1 mutant, they can be restored by increasing the number of recombination intermediates in the double cdkg1-1 fancm-1 mutant. Despite this, recombination intermediates are not correctly resolved leading to the formation of chromosome aggregates at metaphase I. Our results show that CDKG1 is acting early in the recombination process and is necessary to stabilize recombination intermediates. Finally, we show that the effect on recombination is not restricted to meiosis and that CDKG1 is also required for normal levels of DNA damage-induced homologous recombination in somatic tissues.-

拟南芥细胞周期蛋白依赖性激酶G1（CDKG）是雄性减数分裂在高环境温度下重组和突触所必需的。在cdkg1-1突变体中，突触受损，并且I类交叉的数量急剧减少，导致中期I的单价和花粉不育。在这里，我们证明CDKG1对于规范ZMM重组途径中的重组中间体的处理是必需的，而CDKG1的丢失导致II类CO的增加。虽然在cdkg1-1突变体中严重破坏了与I类交叉相关的突触和事件，但可以通过增加双cdkg1-1 fancm-1突变体中重组中间体的数量来恢复突触和事件。尽管如此，重组中间体未正确解析，导致在中期I形成染色体聚集体。我们的结果表明CDKG1在重组过程的早期起作用，对于稳定重组中间体是必需的。最后，我们表明对重组的作用不仅限于减数分裂，而且在体细胞组织中正常水平的DNA损伤诱导的同源重组也需要CDKG1。