DIP2A mutation is associated with abnormal brain development and diseases including dyslexia, autism and Alzheimer's disease. However, the role and the involved mechanisms remain unknown. To study the biological function of DIP2A during mESCs neural differentiation in early neural development, we generated a Dip2a homozygous knockout 46C ESC cell line using CRISPR/Cas9 genome editing technology. The eighth exon of Dip2a gene was replaced with PGK-Puro-P2A-mCherry. This 46C-Dip2a KO cell line offers a useful resource to investigate the molecular mechanisms of DIP2A in the process of cell fate determination, as well as a potential source of building disease mouse model.

DIP2A突变与大脑发育异常和疾病相关，包括阅读障碍，自闭症和阿尔茨海默氏病。但是，其作用和涉及的机制仍然未知。为了研究DIP2A在mESCs神经分化过程中早期神经发育过程中的生物学功能，我们使用CRISPR / Cas9基因组编辑技术生成了Dip2a纯合敲除46C ESC细胞系。Dip2a基因的第八个外显子被PGK-Puro-P2A-mCherry取代。此46C- Dip2a KO细胞系提供了有用的资源，可用于研究DIP2A在细胞命运确定过程中的分子机制，以及建立疾病小鼠模型的潜在来源。