Membranes for guided bone regeneration represent valuable resources, preventing fibroblast infiltration and aiding anatomical bone reconstruction. Nonetheless, available membranes lack bone regenerative capacity, suitable mechanical behavior, or adequate degradation profile. Therefore, to overcome these limitations, this study developed bilayer membranes with a dense layer (dry phase inversion) of PLGA (poly(lactic-co-glycolic acid)):HAp (hydroxyapatite) – 95:05 (wt%) – and an electrospun layer of PLGA and HAp:β-TCP (β-tricalcium phosphate) with ratios of 60:40, 70:30 and 85:15 (wt%), evaluating its mechanical, morphological and in vitro properties. The bilayer membranes displayed adequate interlayer adhesion, dense layer pore size of 4.20 μm and electrospun layer with porosity degree of 38.2%, thus capable of preventing fibroblast infiltration while allowing osteoblast migration and nutrient permeation. They also showed T_g of 82 °C and higher storage modulus, which was constant up to 54.6 °C, characteristics important for membrane implantation and use with no mechanical compromise. In vitro degradation mass loss was only 10% after 60 days, a profile suitable for the application requirement. Membranes with calcium phosphates had better osteoblast attachment, proliferation and migration. Taken together, results indicate the great potential of PLGA/HAp/β-TCP bilayer membranes on bone reconstruction with proper degradation profile, morphology, mechanical behavior and bone regenerative capacity.

引导骨再生的膜代表了宝贵的资源，可防止成纤维细胞浸润并有助于解剖学上的骨骼重建。但是，可用的膜缺乏骨再生能力，合适的机械性能或足够的降解特性。因此，为克​​服这些局限性，本研究开发了一种双层膜，其具有PLGA（聚（乳酸-共-乙醇酸））：HAp（羟基磷灰石）– 95:05（wt％）–的致密层（干相转化）。比例为60：40、70：30和85:15（wt％）的PLGA和HAp：β-TCP（β-磷酸三钙）电纺丝层，评估其力学，形态和体外性能属性。双层膜显示出足够的层间粘附力，致密层孔径为4.20μm，电纺层的孔隙度为38.2％，因此能够防止成纤维细胞浸润，同时允许成骨细胞迁移和营养物渗透。他们还显示出T _g为82°C和更高的储能模量，在高达54.6°C的温度下保持恒定，这对膜植入和使用无机械损伤很重要。体外60天后降解质量损失仅为10％，符合应用要求。含磷酸钙的膜具有更好的成骨细胞附着，增殖和迁移。两者合计，结果表明，PLGA / HAp /β-TCP双层膜具有合适的降解特性，形态，力学行为和骨再生能力，在骨重建方面具有巨大潜力。