2,5‐furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research is to create a CO₂‐driven syntrophic consortium for the catalytic conversion of renewable biomass‐derived 5‐hydroxymethylfurfural (HMF) to FDCA. Sucrose produced from carbon fixation by the engineered Synechococcus elongatus serves as the sole carbon source for the engineered Pseudomonas putida to catalyze the reaction of HMF to FDCA. The yield of FDCA by the consortium reaches around 70% while the conversion of HMF is close to 100%. With further surface engineering to clump the two strains, the FDCA yield is elevated to almost 100% via the specific association between an Src homology 3 (SH3) domain and its ligand. The syntrophic consortium successfully demonstrates its green and cost‐effective characteristics for the conversion of CO₂ and biomass into platform chemicals.

中文翻译：

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通过工程化的拟乳突球菌和恶臭假单胞菌的同养联合体将5-羟甲基糠醛生物转化为2,5-呋喃二甲酸。

2,5-呋喃二甲酸（FDCA）是可以由生物质原料生产的最重要的平台化学品之一。为了使工业FDCA生产的成本与化石制成的塑料兼容，应降低基材的价格和工艺复杂性。这项研究的目的是创建一个由CO ₂驱动的同养性财团，用于将可再生生物质衍生的5-羟甲基糠醛（HMF）催化转化为FDCA。由工程化的Synchococcus elongatus固碳产生的蔗糖是工程化的假单胞菌的唯一碳源催化HMF与FDCA的反应。财团的FDCA收率达到70％左右，而HMF的转化率接近100％。通过进一步的表面工程使这两个菌株聚集在一起，通过Src同源性3（SH3）域与其配体之间的特异性结合，FDCA的产率提高到几乎100％。同养菌联合体成功展示了其绿色环保和经济高效的特性，可将CO ₂和生物质转化为平台化学品。