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Cyclical strain improves artificial equine tendon constructs in vitro.
Journal of Tissue Engineering and Regenerative Medicine ( IF 3.3 ) Pub Date : 2020-03-17 , DOI: 10.1002/term.3030
Francesca Atkinson 1, 2 , Richard Evans 1 , James E Guest 3 , Emma P Bavin 1 , Diogo Cacador 3 , Christopher Holland 2 , Deborah J Guest 1
Affiliation  

Tendon injuries are a common cause of morbidity in humans. They also occur frequently in horses, and the horse provides a relevant, large animal model in which to test novel therapies. To develop novel cell therapies that can aid tendon regeneration and reduce subsequent reinjury rates, the mechanisms that control tendon tissue regeneration and matrix remodelling need to be better understood. Although a range of chemical cues have been explored (growth factors, media etc.), the influence of the mechanical environment on tendon cell culture has yet to be fully elucidated. To mimic the in vivo environment, in this study, we have utilised a novel and affordable, custom‐made bioreactor to apply a cyclical strain to tendon‐like constructs generated in three‐dimensional (3D) culture by equine tenocytes. Dynamic shear analysis (DSA), dynamic scanning calorimetry (DSC) and Fourier‐transform infrared (FTIR) spectroscopy were used to determine the mechanical and chemical properties of the resulting tendon‐like constructs. Our results demonstrate that equine tenocytes exposed to a 10% cyclical strain have an increased amount of collagen gel contraction after 7 and 8 days of culture compared with cells cultured in 3D in the absence of external strain. While all the tendon‐like constructs have a very similar chemical composition to native tendon, the application of strain improves their mechanical properties. We envisage that these results will contribute towards the development of improved biomimetic artificial tendon models for the development of novel strategies for equine regenerative therapies.

中文翻译:

循环应变可改善体外的人工马腱结构。

肌腱损伤是人类发病的常见原因。它们在马中也经常发生,并且马提供了相关的大型动物模型来测试新颖的疗法。为了开发有助于肌腱再生并减少后续再损伤率的新型细胞疗法,需要更好地理解控制肌腱组织再生和基质重塑的机制。尽管已探索了一系列化学线索(生长因子,培养基等),但尚未完全阐明机械环境对肌腱细胞培养的影响。为了模拟体内环境,在这项研究中,我们利用了一种新型且价格合理的定制生物反应器,将周期性应变应用于马腱细胞在三维(3D)培养中生成的肌腱样结构。动态剪切分析(DSA),动态扫描量热法(DSC)和傅立叶变换红外光谱(FTIR)光谱用于确定所得肌腱样结构的机械和化学性质。我们的结果表明,与没有外部应变的3D培养细胞相比,暴露于10%周期性应变的马腱细胞在培养7天和8天后具有增加的胶原凝胶收缩量。尽管所有类似肌腱的构造物都具有与天然肌腱非常相似的化学组成,但施加应变可改善其机械性能。我们设想这些结果将有助于改进仿生人工肌腱模型的开发,以发展马再生疗法的新策略。
更新日期:2020-03-17
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