Abstract

Objective

To evaluate total absorbance, planktonic growth, biofilm formation, viability, metabolic activity, and pH of Streptococcus mutans UA159 cultures when different dilutions of Stevia rebaudiana Bertoni were applied and to determine the minimum inhibitory concentration (MIC) and the minimum biofilm inhibitory concentration (MBIC) of Stevia on S. mutans.

Materials and methods

The effects of different dilutions of Stevia (0–400 mg/ml) on S. mutans total growth, planktonic growth, biofilm formation, viability, metabolic activity, and pH during a 72-h growth period were evaluated in this in vitro study. A stock solution was prepared by mixing 10 ml of tryptic soy broth (TSB) supplemented with 1% sucrose (TSBS) and 4 g of Stevia.

Results

S. mutans total growth and biofilm formation decreased with reduced concentrations of Stevia. Furthermore, the MIC was 25 mg/ml and the MBIC was 6.25 mg/ml. Complete eradication of S. mutans was not observed with any of the Stevia concentrations. Planktonic growth of S. mutans was not repressed by high concentrations of Stevia and most of the Stevia concentrations generated an increased pH.

Conclusion

Because Stevia reduces biofilm and acid production, Stevia can be considered a non-cariogenic sweetener.

Clinical relevance

This study confirms the anticariogenic effect of Stevia, like it has been previously reported, but more studies on the most effective concentration are needed, and in the present study, the minimum inhibitory concentration (MIC) and the minimum biofilm inhibitory concentration (MBIC) was determined in the presence of sucrose. Additionally, this is the first study to evaluate the effect of Stevia on S. mutans metabolic activity.

中文翻译：

---

不同浓度的甜叶菊甜叶菊在蔗糖中变形链球菌的生长和活力。

摘要

目的

当使用不同稀释度的甜叶菊甜菜评估不同的变形链球菌UA159培养物的总吸收，浮游生长，生物膜形成，活力，代谢活性和pH值，并确定最小抑菌浓度（MIC）和最小生物膜抑菌浓度（MBIC）甜叶菊对变形链球菌）。

材料和方法

在这项体外研究中，评估了72倍生长期内，不同稀释度的甜叶菊（0–400 mg / ml）对变形链球菌总生长，浮游生物生长，生物膜形成，活力，代谢活性和pH的影响。通过将10 ml补充了1％蔗糖（TSBS）的胰蛋白酶大豆肉汤（TSB）和4 g甜叶菊混合来制备储备溶液。

结果

随着甜叶菊浓度降低，变形链球菌的总生长和生物膜形成减少。此外，MIC为25mg / ml，MBIC为6.25mg / ml。在任何甜菊浓度下均未观察到完全消除变形链球菌。高浓度的甜叶菊不能抑制变形链球菌的浮游生物生长，大多数甜叶菊浓度都可以增加pH。

结论

由于甜菊降低了生物膜和酸的产生，因此甜菊可以被认为是非致龋性甜味剂。

临床相关性

这项研究证实了甜叶菊的抗癌作用，就像先前报道的一样，但是需要对最有效浓度进行更多的研究，在本研究中，最小抑菌浓度（MIC）和最小生物膜抑菌浓度（MBIC）为在蔗糖存在下测定。此外，这是第一个评估甜叶菊对变形链球菌代谢活性影响的研究。