Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease with a mortality rate of up to 50% in humans. To avoid safety concerns associated with the use of live virus in virus neutralization assays and to detect human serum neutralizing antibodies, we prepared lentiviral particles containing the CCHF glycoprotein (lenti-CCHFV-GP). Incorporation of the GP into the lentiviral particle was confirmed by electron microscopy and Western blotting. Lenti-CCHFV-GP was found to be able to infect a wide range of cell lines, including BHK-21, HeLa, HepG2, and AsPC-1 cells. In addition, lenti-CCHFV-GP was successfully used as an alternative to CCHFV for the detection of neutralizing antibodies. Sera collected from CCHF survivors neutralized lenti-CCHFV-GP particles in a dose-dependent manner. Our results suggest that the lenti-CCHFV-GP pseudovirus can be used as a safe tool for neutralization assays in low-containment laboratories.

中文翻译：

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展示克里米亚-刚果出血热病毒糖蛋白的慢病毒系统的有效生产揭示了广泛的细胞敏感性和中和能力。

克里米亚-刚果出血热（CCHF）是由tick传播的疾病，人类的死亡率高达50％。为避免与在病毒中和试验中使用活病毒有关的安全问题并检测人血清中和抗体，我们制备了包含CCHF糖蛋白（lenti-CCHFV-GP）的慢病毒颗粒。通过电子显微镜和蛋白质印迹证实了GP掺入慢病毒颗粒。发现慢病毒-CCHFV-GP能够感染多种细胞系，包括BHK-21，HeLa，HepG2和AsPC-1细胞。此外，慢病毒-CCHFV-GP已成功用作CCHFV的替代品，用于检测中和抗体。从CCHF幸存者收集的血清以剂量依赖性方式中和了慢病毒CCHFV-GP颗粒。