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Akkermansia muciniphila Prevents Fatty Liver Disease, Decreases Serum Triglycerides, and Maintains Gut Homeostasis
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-03-18
Kim, S., Lee, Y., Kim, Y., Seo, Y., Lee, H., Ha, J., Lee, J., Choi, Y., Oh, H., Yoon, Y.

The objective of this study was to elucidate the effect of intestinal Akkermansia muciniphila bacteria on fatty liver disease. Five-week-old C57BL/6N mice were administered either phosphate-buffered saline (PBS; control) or A. muciniphila at 108 to 109 CFU/ml, and were fed either a 45% fat diet (high-fat diet [HFD]) or a 10% fat diet (normal diet [ND]) for 10 weeks. After 10 weeks, the mice were euthanized, and blood and tissue samples, including adipose tissue, cecum, liver, and brain, were immediately collected. Biochemical and histological analyses were conducted, and the expression levels of related factors were compared to determine the antiobesity effects of Akkermansia muciniphila. The gut microbiome was analyzed in fecal samples. Oral administration of A. muciniphila significantly (P < 0.05) lowered serum triglyceride (TG) and alanine aminotransferase (ALT) levels in obese mice. Compared to the non-A. muciniphila-treated group, the expression of SREBP (regulator of TG synthesis in liver tissue) was decreased in the A. muciniphila-treated group. The expression of IL-6 in the liver of obese mice was decreased following the administration of A. muciniphila. Furthermore, alterations in the ratio of Firmicutes to Bacteroidetes and the decrease in bacterial diversity caused by the HFD were restored upon the administration of A. muciniphila. These results indicate that A. muciniphila prevents fatty liver disease in obese mice by regulating TG synthesis in the liver and maintaining gut homeostasis.

IMPORTANCE This study investigated the effect of Akkermansia muciniphila on fatty liver disease. Although some research about the effects of A. muciniphila on host health has been published, study of the relationship between A. muciniphila administration and fatty liver, as well as changes in the gut microbiota, has not been conducted. In this study, we demonstrated that A. muciniphila prevented fatty liver disease by regulation of the expression of genes that regulate fat synthesis and inflammation in the liver.



中文翻译:

Akkermansia muciniphila可预防脂肪肝疾病,减少血清甘油三酸酯并维持肠道稳态

本研究的目的是阐明肠黏膜阿克曼氏菌对脂肪肝的影响。给五周大的C57BL / 6N小鼠以10 8至10 9 CFU / ml的剂量施用磷酸盐缓冲盐水(PBS;对照)或黏液曲霉,并饲喂45%的脂肪饮食(高脂饮食[ HFD])或10%脂肪饮食(正常饮食[ND])持续10周。10周后,对小鼠实施安乐死,并立即收集血液和组织样本,包括脂肪组织,盲肠,肝和脑。进行了生化和组织学分析,并比较了相关因子的表达水平,以确定粘蛋白阿克曼(Akkermansia muciniphila)的抗肥胖作用。在粪便样品中分析了肠道微生物组。口服黏液曲霉可显着降低肥胖小鼠的血清甘油三酸酯(TG)和丙氨酸氨基转移酶(ALT)(P <0.05)。与非A. muciniphila治疗组相比,SREBP(肝组织中TG合成的调节剂)的表达在A. muciniphila治疗组中降低。给予粘液曲霉后,肥胖小鼠肝脏中IL-6的表达降低。此外,硬顶菌拟杆菌比率的改变和HFD引起的细菌多样性的减少恢复给予黏液曲霉。这些结果表明黏液曲霉可通过调节肝脏中的TG合成并维持肠道稳态预防肥胖小鼠的脂肪肝疾病。

重要性这项研究调查的影响Akkermansia muciniphila对脂肪肝。尽管已经发表了一些关于黏液曲霉对宿主健康的影响的研究,但尚未进行关于黏液曲霉的给药与脂肪肝之间关系以及肠道菌群变化的研究。在这项研究中,我们证明了A. muciniphila通过调节调节肝脏脂肪合成和炎症的基因表达来预防脂肪肝。

更新日期:2020-03-19
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