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Surfactant exopolysaccharide of Ochrobactrum pseudintermedium C1 has antibacterial potential: Its bio-medical applications in vitro.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.micres.2020.126466
Dipanjan Sengupta 1 , Sriparna Datta 1 , Dipa Biswas 1
Affiliation  

Since the advent of biologics in human welfare various bio-molecules have been explored. Different bacterial exopolysaccharides have proved their worth in many industrial and commercial applications. In this perspective, while exploring a surfactant exopolysaccharide of Ochrobactrum pseudintermedium C1, it is strikingly observed that it possesses a potent antibacterial property which encourages its bio-medical applications. Following isolation and purification of the said exopolysaccharide, its structural configuration and functional attributes are studied by several analytical procedures involving FTIR, 13C- NMR, CHN-analysis, estimation of zeta potential, XRD-study and digital tensiometry. When treated with pathological samples in vitro, it distinctly elicits its antibacterial property by exhibiting a characteristic zone of inhibition. Combined with a standard antibiotic (like ciprofloxacin), it enhances the action of antibiotic also. Mechanism of its antibacterial action is evaluated by crystal violet entrapment assay with UV–vis spectrophotometry, bacterial cell viability assay by trypan blue staining and SEM study. Results show that its basic surfactant property, anionic character, crystalline nature and scaffolding architecture are supposed to facilitate its antibacterial property which is manifested by its capability of disrupting bacterial cell envelope causing eventual cell death. In the current global scenario, an increasing threat of antibiotic resistance is prevailing due to their indiscriminate use. If used as an adjuvant with a judicious dose of antibiotic, this bio-molecule might play a significant role in bio-medicine to combat such threat.



中文翻译:

假ch骨C1表面活性剂胞外多糖具有抗菌潜力:其在体外的生物医学应用。

自从生物制剂出现在人类福祉中以来,已经探索了各种生物分子。不同的细菌胞外多糖已在许多工业和商业应用中证明了其价值。从这个角度出发,在探索假牙菌C1的表面活性剂胞外多糖时,令人惊讶地观察到它具有有效的抗菌特性,可促进其生物医学应用。在分离和纯化所述胞外多糖之后,通过几种分析程序,包括FTIR,13 C-NMR,CHN分析,ζ电势估计,XRD研究和数字张力测定法研究其结构构型和功能特性。体外用病理样本处理时,它通过表现出特征性的抑制区域而明显地激发了其抗菌性能。与标准抗生素(如环丙沙星)结合使用,还可增强抗生素的作用。通过紫外可见分光光度法测定结晶紫包埋法,台盼蓝染色法和SEM研究细菌细胞活力,从而评估了其抗菌作用的机制。结果表明,其基本的表面活性剂性质,阴离子特性,结晶性质和支架结构被认为促进了其抗菌性质,这由其破坏细菌细胞包膜导致最终细胞死亡的能力来证明。在当前的全球情况下,由于不加选择地使用抗生素,其耐药性威胁日益增加。如果用作明智剂量的抗生素佐剂,

更新日期:2020-03-17
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