Filamentous fungi are well known for producing secondary metabolites applied in various industrial segments. Among these, lovastatin and itaconic acid, produced by Aspergillus terreus, have applications in the pharmaceutical and chemical industries. Lovastatin is primarily used for the control of hypercholesterolemia, while itaconic acid is a building block for the production of synthetic fibers, coating adhesives, among others. In this study, for the first time, 35 strains of Aspergillus sp. from four Brazilian culture collections were evaluated for lovastatin and itaconic acid production and compared to a reference strain, ATCC 20542. From an initial screening, the strains ATCC 20542, URM 224, URM1876, URM 5061, URM 5254, URM 5256, URM 5650, and URM 5961 were selected for genomic comparison. Among tested strains, the locus corresponding to the lovastatin genomic cluster was assembled, showing that all genes essential for lovastatin biosynthesis were present in producing URM 5961 and URM 5650 strains, with 100% and 98,5% similarity to ATCC 20542, respectively. However, in the no producing URM 1876, URM 224, URM 5254, URM 5061, and URM 5256 strains, this cluster was either fragmented or missing. Among the 35 strains evaluated for itaconic acid production in this study, only three strains had titers above 0.5 g/L, 16 strains had production below 0.5 g/L, and the remaining 18 strains had no production, with the highest production of itaconic acid observed in the URM 5254 strain with 2.2 g/L. The essential genes for itaconic acid production, mttA, cadA msfA were also mapped, where all three genes linked to itaconic acid production were found in a single contig in the assembly of each strain. In contrast to lovastatin loci, there is no correlation between the level of itaconic acid production and genetic polymorphisms in the genes associated with its biosynthesis.

丝状真菌是众所周知的用于产生应用于各种工业领域的次级代谢产物。其中，由曲霉属曲霉生产的洛伐他汀和衣康酸已用于制药和化学工业。洛伐他汀主要用于控制高胆固醇血症，而衣康酸则是生产合成纤维，涂料粘合剂等的基础。在这项研究中，首次有35株曲霉sp。对来自四个巴西培养物保藏中心的洛伐他汀和衣康酸的生产进行了评估，并将其与参考菌株ATCC 20542进行了比较。通过初步筛选，菌株ATCC 20542，URM 224，URM1876，URM 5061，URM 5254，URM 5256，URM 5650，选择了URM 5961和URM 5961进行基因组比较。在测试的菌株中，组装了对应于洛伐他汀基因组簇的基因座，显示出洛伐他汀生物合成必需的所有基因均存在于生产URM 5961和URM 5650菌株中，分别与ATCC 20542相似性为100％和98.5％。但是，在无生产的URM 1876，URM 224，URM 5254，URM 5061和URM 5256菌株中，该簇断裂或缺失。在这项研究中评估的35种衣康酸菌株中，只有3株的滴度高于0.5 g / L，16株菌株的产量低于0.5 g / L，其余18株菌株没有产量，在URM 5254菌株中，衣康酸的最高产量为2.2 g / L。衣康酸生产的必需基因还绘制了mtt A，cad A msf A的图谱，其中在每个菌株的装配体的单个重叠群中发现了与衣康酸生产相关的所有三个基因。与洛伐他汀基因座相反，衣康酸的产生水平与与其生物合成相关的基因中的遗传多态性之间没有相关性。