The majority of amyotrophic lateral sclerosis are sporadic (sALS) with no familial history or known genetic association, therefore a large cohort of disease models are required to identify common mechanisms or to test therapeutic interventions. Here we generated twelve induced pluripotent stem cell (iPSC) lines from human dermal fibroblasts of two healthy individuals and two sALS patients lacking common ALS mutations, using non-integrational Sendai virus expressing reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC. The iPSC lines highly expressed pluripotency markers could be spontaneously differentiated into three embryonic germ layers, with no gross chromosomal aberrations or specific copy number variations.

大多数肌萎缩侧索硬化症是散发性的 (sALS)，没有家族史或已知的遗传关联，因此需要大量疾病模型来确定常见机制或测试治疗干预措施。在这里，我们使用表达重编程因子 OCT3/4、KLF4、SOX2 和 c-MYC 的非整合仙台病毒，从两个健康个体和两个缺乏常见 ALS 突变的 sALS 患者的人皮肤成纤维细胞中生成了 12 个诱导多能干细胞 (iPSC) 系。iPSC 系高度表达的多能性标记可以自发分化成三个胚胎胚层，没有明显的染色体畸变或特定的拷贝数变化。