Lon and ClpXP are ATP‐dependent proteases that help maintain human mitochondrial integrity. The two have overlapping substrate specificity, making it difficult to distinguish their activities. In this study, a fluorogenic peptide substrate was designed and shown to be selective in monitoring ClpXP peptidase activity in isolated HeLa cell lysate.

中文翻译：

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人类线粒体ATP依赖性蛋白酶复合物ClpXP的选择性荧光肽底物。

Lon和ClpXP是依赖ATP的蛋白酶，有助于维持人类线粒体的完整性。两者具有重叠的底物特异性，因此很难区分它们的活性。在这项研究中，设计了一种荧光肽底物，并证明在监测分离的HeLa细胞裂解物中ClpXP肽酶活性方面具有选择性。