Self‐service : We review recent developments in chemical probes for self‐labeling protein tags, focusing on the HaloTag and SNAP‐tag systems. Content includes the recently developed small‐molecule probes for fluorescence microscopy and several methodologies that perturb or manipulate biological processes in live cells. Future developments in self‐labeling protein tags should enable methods to address previously inaccessible biological questions.

中文翻译：

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使用 HaloTag 和 SNAP-Tag 技术可视化和操纵生物过程。

自助服务：我们回顾了用于自标记蛋白质标签的化学探针的最新发展，重点关注 HaloTag 和 SNAP 标签系统。内容包括最近开发的用于荧光显微镜的小分子探针以及干扰或操纵活细胞中生物过程的几种方法。自标记蛋白质标签的未来发展应该能够解决以前无法解决的生物学问题。