当前位置: X-MOL 学术PLOS Genet. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis.
PLOS Genetics ( IF 4.5 ) Pub Date : 2020-03-16 , DOI: 10.1371/journal.pgen.1008275
Heinrich Schäfer 1, 2 , Bertrand Beckert 3 , Christian K Frese 2 , Wieland Steinchen 4 , Aaron M Nuss 5 , Michael Beckstette 5 , Ingo Hantke 1 , Kristina Driller 2 , Petra Sudzinová 6 , Libor Krásný 6 , Volkhard Kaever 7 , Petra Dersch 5, 8 , Gert Bange 4 , Daniel N Wilson 3 , Kürşad Turgay 1, 2
Affiliation  

Bacillus subtilis cells are well suited to study how bacteria sense and adapt to proteotoxic stress such as heat, since temperature fluctuations are a major challenge to soil-dwelling bacteria. Here, we show that the alarmones (p)ppGpp, well known second messengers of nutrient starvation, are also involved in the heat stress response as well as the development of thermo-resistance. Upon heat-shock, intracellular levels of (p)ppGpp rise in a rapid but transient manner. The heat-induced (p)ppGpp is primarily produced by the ribosome-associated alarmone synthetase Rel, while the small alarmone synthetases RelP and RelQ seem not to be involved. Furthermore, our study shows that the generated (p)ppGpp pulse primarily acts at the level of translation, and only specific genes are regulated at the transcriptional level. These include the down-regulation of some translation-related genes and the up-regulation of hpf, encoding the ribosome-protecting hibernation-promoting factor. In addition, the alarmones appear to interact with the activity of the stress transcription factor Spx during heat stress. Taken together, our study suggests that (p)ppGpp modulates the translational capacity at elevated temperatures and thereby allows B. subtilis cells to respond to proteotoxic stress, not only by raising the cellular repair capacity, but also by decreasing translation to concurrently reduce the protein load on the cellular protein quality control system.



中文翻译:

警铃(p)ppGpp是枯草芽孢杆菌热休克反应的一部分。

枯草芽孢杆菌由于温度波动是居住在土壤中的细菌的主要挑战,因此细胞非常适合研究细菌如何感测和适应诸如热的蛋白毒性胁迫。在这里,我们显示出警报素(p)ppGpp,众所周知的营养饥饿的第二信使,也参与了热应激反应以及耐热性的发展。受到热冲击后,细胞内(p)ppGpp的水平以快速但短暂的方式上升。热诱导的(p)ppGpp主要是由核糖体相关的警报合成酶Rel产生的,而小的警报合成酶RelP和RelQ似乎不参与其中。此外,我们的研究表明,生成的(p)ppGpp脉冲主要作用于翻译水平,只有特定的基因在转录水平受调控。hpf,编码保护核糖体的冬眠促进因子。另外,在热应激期间,警号似乎与应激转录因子Spx的活性相互作用。总之,我们的研究表明,(p)的调制ppGpp的在升高的温度下的平移容量,从而允许枯草杆菌细胞不仅通过提高细胞修复能力,而且通过减少翻译以同时减少细胞蛋白质质量控​​制系统上的蛋白质负载,从而对蛋白毒性应激作出反应。

更新日期:2020-04-06
down
wechat
bug