In previous studies of ionic liquid (IL) tolerance of numerous species of ascomycetous yeasts, two strains of Wickerhamomyces ciferrii and Galactomyces candidus had unusually high tolerance in media containing up to 5% (w/v) of the 1-ethyl-3-methylimidazolium acetate ([C₂C₁Im][OAc]). The study aimed at investigating whether additional strains of these species, and additional species in the Dipodascaceae family, also possess IL tolerance, and to compare sensitivity to the acetate and chloride versions of the ionic liquid. Fifty five yeast strains in the family Dipodascaceae, which encompasses genera Galactomyces, Geotrichum, and Dipodascus, and seven yeast strains of species Wickerhamomyces ciferrii were tested for ability to grow in laboratory medium containing no IL, 242 mM [C₂C₁Im][OAc], or 242 mM [C₂C₁Im]Cl, and in IL-pretreated switchgrass hydrolysate. Many yeasts exhibited tolerance of one or both ILs, with higher tolerance of the chloride anion than of the acetate anion. Different strains of the same species exhibited varying degrees of IL tolerance. Galactomyces candidus, UCDFSTs 52–260, and 50–64, had exceptionally robust growth in [C₂C₁Im][OAc], and also grew well in the switchgrass hydrolysate. Identification of IL tolerant and IL resistant yeast strains will facilitate studies of the mechanism of IL tolerance, which could include superior efflux, metabolism or exclusion.

在以前对多种子囊酵母的离子液体（IL）耐受性的研究中，两个Wickerhamomyces ciferrii和Galactomyces candidus菌株在含有高达5％（w / v）1-乙基-3-甲基咪唑鎓的培养基中具有异常高的耐受性乙酸盐（[C ₂ C ₁ Im] [OAc]）。这项研究旨在调查这些物种的其他菌株以及双足纲科中的其他物种是否也具有IL耐受性，并比较对离子液体的乙酸盐和氯化物版本的敏感性。在家庭中的五十五酵母菌株Dipodascaceae，其涵盖属Galactomyces，地霉属，和Dipodascus种，和七个酵母菌株Wickerhamomyces赤酵母在不含IL，242毫[C实验室培养基中生长的能力进行了测试₂ ç ₁ IM] [OAC]，或242 mM的[C ₂ C ^ ₁ IM] Cl和在IL预处理的柳枝hydro水解产物。许多酵母菌表现出一种或两种IL的耐受性，其中氯离子的耐受性高于乙酸根阴离子。同一物种的不同菌株表现出不同程度的IL耐受性。Galactomyces candidus的，UCDFSTs 52-260，和50-64，不得不在异常生长健壮[C ₂ C ^ ₁Im] [OAc]，并且在柳枝hydro水解液中也生长良好。IL耐受和IL抗性酵母菌株的鉴定将有助于研究IL耐受的机制，其中可能包括优异的外排，代谢或排斥。