Hydrogen sulfide (H2S), a plant gasotransmitter, functions in the plant response to cadmium (Cd) stress, implying a role for cysteine desulfhydrase in producing H2S in this process. Whether d -CYSTEINE DESULFHYDRASE (DCD) acts in the plant Cd response remains to be identified, and if it does, how DCD is regulated in this process is also unknown. Here, we report that DCD-mediated H2S production enhances plant Cd tolerance in Arabidopsis (Arabidopsis thaliana). When subjected to Cd stress, a dcd mutant accumulated more Cd and reactive oxygen species and showed increased Cd sensitivity, whereas transgenic lines overexpressing DCD had decreased Cd and reactive oxygen species levels and were more tolerant to Cd stress compared with wild-type plants. Furthermore, the expression of DCD was stimulated by Cd stress, and this up-regulation was mediated by a Cd-induced transcription factor, WRKY13, which bound to the DCD promoter. Consistently, the higher Cd sensitivity of the wrky13-3 mutant was rescued by the overexpression of DCD Together, our results demonstrate that Cd-induced WRKY13 activates DCD expression to increase the production of H2S, leading to higher Cd tolerance in plants.

中文翻译：

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WRKY13通过促进D-半胱氨酸脱硫酶和硫化氢的产生来提高镉的耐受性。

硫化氢（H2S）是植物的一种气体递质，在植物对镉（Cd）胁迫的响应中起作用，这暗示半胱氨酸脱硫酶在此过程中产生H2S的作用。d-半胱氨酸脱硫酶（DCD）是否在植物Cd响应中起作用尚待确定，如果确实如此，在此过程中如何调节DCD也是未知的。在这里，我们报告DCD介导的H2S生产增强了拟南芥（Arabidopsis thaliana）中植物Cd的耐受性。受到Cd胁迫时，dcd突变体积累了更多的Cd和活性氧，并显示了对Cd的敏感性，而过表达DCD的转基因品系与野生型植物相比，其Cd和活性氧的含量降低，并且对Cd胁迫的耐受性更高。此外，Cd刺激了DCD的表达，并且这种上调是由Cd诱导的转录因子WRKY13介导的，该转录因子与DCD启动子结合。一致地，通过DCD的过量表达挽救了wrky13-3突变体的更高Cd敏感性。我们的结果表明，Cd诱导的WRKY13激活DCD表达以增加H2S的产生，从而导致植物对Cd的耐受性更高。