Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.carbpol.2020.116181 Qinghua Liu , Zuobin Zhu , Mengmeng Wang , Yuechen Wang , Peng Zhang , Hao Wang , Mengyu Liang , Ying Li , Bin Deng , Daoquan Tang , Robert G. Gilbert , Liang Wang
Glycogen, a glucose homopolymer with many glucose chains, is the primary blood-sugar reservoir in many organisms. It comprises β particles (∼20 nm) which can bind together to form large α particles with a rosette morphology. When dimethyl sulfoxide (DMSO) is added to glycogen from diabetic livers, α particles break apart to β particles (‘fragility’), possibly due to H-bond disruption; this is not seen in healthy livers. Glycogen α and β particles, and α-particle fragility, are observed in mammals and bacteria, and are examined here in the worm Caenorhabditis elegans, with glycogen from two C. elegans strains, cultured in normal and high-glucose conditions. There were mainly β particles, with some large α particles. Most particles were fragile in DMSO. Growing in a high-glucose medium results in more long chains and more fragility, consistent with previous observations in diabetic animal models. Why high glucose levels facilitate fragility is worthy of further investigation.
中文翻译:
线虫秀丽隐杆线虫糖原分子结构的表征
糖原是一种具有许多葡萄糖链的葡萄糖均聚物,是许多生物体中主要的血糖储存库。它包含β粒子(约20 nm),可以结合在一起形成具有玫瑰花形形态的大α粒子。当将二甲基亚砜(DMSO)添加到糖尿病肝脏的糖原中时,α颗粒会分解为β颗粒(“易碎性”),这可能是由于H键断裂所致;这在健康的肝脏中是看不到的。在哺乳动物和细菌中观察到了糖原α和β颗粒以及α颗粒的脆性,并在线虫秀丽隐杆线虫中进行了检查,其中糖原来自两个秀丽隐杆线虫在正常和高葡萄糖条件下培养的菌株。主要有β粒子,还有一些大的α粒子。多数颗粒在DMSO中易碎。在高葡萄糖培养基中生长会导致更长的链和更大的脆弱性,这与之前在糖尿病动物模型中的观察结果一致。为什么高葡萄糖水平会促进脆性值得进一步研究。