The application of Campylobacter specific bacteriophages appears as a promising food safety tool for the biocontrol of this pathogen in the poultry meat production chain. However, their isolation is a complicated challenge since their occurrence appears to be low. This work assessed the efficiency of seven protocols for recovering Campylobacter phages from chicken skin samples inoculated at phage loads from 5.0 × 101 to 5.0 × 106 PFU/g. The enrichment of chicken skin in selective Bolton broth containing target isolates was the most efficient procedure, showing a low detection limit of 5.0 × 101 PFU/g and high recovery rates of up to 560%. This method's effectiveness increased as phage concentration decreased, showing its suitability for phage isolation. When this method was applied to isolate new Campylobacter phages from retail chicken skin, a total of 280 phages were recovered achieving an isolation success rate of 257%. From the 109 samples 68 resulted phage positive (62%). Chicken skin could be, therefore, considered a rich source in Campylobacter phages. This method is a simple, reproducible and efficient approach for the successful isolation of both group II and III Campylobacter specific bacteriophages, which could be helpful for the enhancement of food safety by reducing this pathogen contamination in broiler meat.

中文翻译：

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从鸡皮中高效分离弯曲杆菌细菌，分析几种分离方案。

弯曲杆菌特异性噬菌体的应用似乎是在禽肉生产链中对该病原体进行生物防治的有前途的食品安全工具。但是，由于它们的出现率很低，因此将它们隔离是一个复杂的挑战。这项工作评估了从以5.0×101至5.0×106 PFU / g的噬菌体负荷接种的鸡皮样品中回收弯曲杆菌噬菌体的七种方案的效率。最有效的方法是在含有目标分离物的精选博尔顿肉汤中富集鸡皮，该方法的最低检出限为5.0×101 PFU / g，回收率高达560％。该方法的有效性随着噬菌体浓度的降低而增加，表明其适用于噬菌体分离。当此方法用于从零售鸡皮中分离出新的弯曲杆菌噬菌体时，总共回收了280个噬菌体，分离成功率为257％。从109个样品中获得68个噬菌体阳性（62％）。因此，鸡皮可以被认为是弯曲杆菌噬菌体的丰富来源。该方法是成功分离第二组和第三组弯曲杆菌特异性噬菌体的简单，可重复且有效的方法，通过减少肉鸡中这种病原体的污染，可能有助于提高食品安全性。