Modification of micellar liquid chromatography (MLC) with a natural deep eutectic solvent (NADES) and butanol was effectively performed for analysis of four crucial cardiovascular drugs namely aspirin, atorvastatin, metformin, and metoprolol. The multivariate software tools were employed for screening and optimizing the primary experimental parameters including sodium dodecyl sulphate concentration ([SDS]) as well as the volume percentages of NADES, butanol, and glacial acetic acid (GAC). By using the desirability function, the optimal framework of the mobile phase was obtained at volume ratio of 83:10:3.5:3.5, respectively, for SDS (0.09 mol L-1), butanol, NADES, and GAC. Moreover, the curvature and effect plots were applied for evaluating the robustness of the procedure. At the optimal conditions, the drugs were excellently separated within a total analysis time of 12 min when the flow rate of the mobile phase was 1 mL min-1. To certify the performance of the developed procedure, the Food and Drug Administration guidelines for bioanalytical analysis have been implemented. The designed MLC system was successfully utilized for the quantification of the drugs in urine and plasma samples.

中文翻译：

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使用天然的深共熔溶剂作为可持续的流动相添加剂，以改善通过胶束液相色谱分离四种关键心血管药物的效果。

用天然的深共熔溶剂（NADES）和丁醇对胶束液相色谱（MLC）进行了修饰，可以有效地分析四种重要的心血管药物，即阿司匹林，阿托伐他汀，二甲双胍和美托洛尔。多元软件工具用于筛选和优化主要的实验参数，包括十二烷基硫酸钠浓度（[SDS]）以及NADES，丁醇和冰醋酸（GAC）的体积百分比。通过使用期望函数，对于SDS（0.09 mol L-1），丁醇，NADES和GAC，分别以83：10：3​​.5：3.5的体积比获得了最佳的流动相框架。此外，曲率和效果图用于评估该过程的鲁棒性。在最佳条件下 当流动相的流速为1 mL min-1时，药物在12分钟的总分析时间内被很好地分离。为了证明已开发程序的性能，已实施了食品药品监督管理局生物分析分析指南。设计的MLC系统已成功用于定量尿液和血浆样品中的药物。