当前位置: X-MOL 学术Spectrochim. Acta. A Mol. Biomol. Spectrosc. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Facile preparation of FITC-modified silicon nanodots for ratiometric pH sensing and imaging
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.4 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.saa.2020.118276
Yanan Zhang , Dajun Hou , Xueli Yu

A ratiometric fluorescent pH sensor was facilely constructed by covalent modification of amino-terminated silicon nanodots (SiND) with pH-sensitive fluorescein isothiocyanate (FITC). After optimization, the SiND-FITC(40:1) material with a SiND:FITC initial mass ratio of 40:1 was selected for the sensing of hydrogen ions. It was observed that the material inherits the unique features of SiND and FITC, and there is significant improvement of SiND acid−base stability, which is a favorable factor in terms of providing fluorescence reference signal. The SiND-FITC(40:1) material displays not only high pH sensitivity, but also good stability and anti-interference ability, and the response process is highly reversible. Deploying the SiND-FITC(40:1) material, we have made available a simple, sensitive, and precise approach for pH sensing. In aqueous solutions, the I517/I466 fluorescence intensity ratio of SiND-FITC(40:1) increases linearly in the pH range of 5.40–7.76. This dual emission nanosensor was successfully applied for pH sensing and cellular fluorescence imaging.



中文翻译:

方便地制备FITC修饰的硅纳米点以进行比例pH传感和成像

通过用pH敏感的异硫氰酸荧光素(FITC)对氨基末端的硅纳米点(SiND)进行共价修饰,可以轻松构建比例荧光pH传感器。经过优化后,选择SiND:FITC初始质量比为40:1的SiND-FITC(40:1)材料用于感测氢离子。观察到该材料继承了SiND和FITC的独特功能,并且显着提高了SiND酸碱稳定性,这在提供荧光参考信号方面是一个有利因素。SiND-FITC(40:1)材料不仅显示出高pH敏感性,而且还具有良好的稳定性和抗干扰能力,并且响应过程具有高度可逆性。部署SiND-FITC(40:1)材料后,我们提供了一种简单,灵敏且精确的pH传感方法。在5.40–7.76的pH范围内,SiND-FITC(40:1)的517 / I 466荧光强度比呈线性增加。这种双发射纳米传感器已成功应用于pH传感和细胞荧光成像。

更新日期:2020-03-19
down
wechat
bug