A novel electrochemical sensor for a neural cell adhesion molecule (CD56) was constructed by glycosyl imprinting. A sandwich-like multi-signal generation strategy was first proposed in glycosyl imprinting sensors via boric acid affinity. Glycosyl-imprinted polymers were formed by electro-polymerization with poly-sialic acid (PolySia) as a template molecule and p-aminobenzeneboronic (p-ABA) acid as a functional monomer. Methods such as scanning electron microscope (SEM), Fourier transform infrared spectrum (FT-IR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were used to characterize the successful formation of imprinted membranes. Confirmed by both simulation calculation and experimental results, a signal-amplified effect based on macromolecules was introduced for the first time. After re-absorption, aminobenzene borate was linked to the surface of the sensor by boric acid affinity due to the rich hexadoxyl structure of the CD56-terminal chain as a signal probe. Under optimal conditions, the detection limit of the sensor is as low as 0.47 ng/L, and it can be successfully applied to the detection of CD56 in human serum.

通过糖基印迹构建了神经细胞粘附分子（CD56）的新型电化学传感器。首先通过硼酸亲和力在糖基印迹传感器中提出了一种类似三明治的多信号生成策略。糖基印迹聚合物是通过以聚唾液酸（PolySia）为模板分子和对氨基苯硼酸（p-ABA）酸作为功能单体。使用扫描电子显微镜（SEM），傅里叶变换红外光谱（FT-IR），循环伏安法（CV）和电化学阻抗谱（EIS）等方法来表征印迹膜的成功形成。经过仿真计算和实验结果的证实，首次引入了基于大分子的信号放大效应。重新吸收后，由于作为信号探针的CD56末端链的六羟基结构丰富，氨基硼酸硼酸盐通过硼酸亲和力与传感器表面连接。在最佳条件下，该传感器的检出限低至0.47 ng / L，可成功应用于人血清中CD56的检测。