Inflammatory cytokines contribute to proatherogenic changes in lipid metabolism by reduction of HDL-cholesterol (HDL-C) levels, impairment of its antiinflammatory and antioxidant functions. Therefore, the protective actions of HDL-C can be limited in chronic inflammatory diseases such as multiple sclerosis (MS). The aim of this study was to assess the association between lipoprotein subfractions and inflammatory status in early stages of multiple sclerosis. Polyacrylamide gel electrophoresis Lipoprint© System was used for lipoprotein profile analysis in 19 newly diagnosed MS patients, and in matched 19 healthy controls. Serum levels of interleukin (IL) 1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, interferon-γ and TNF-α were measured by multiplex bead assay. Concentrations of the measured cytokines and lipoprotein subclasses were comparable between MS patients and controls. Male, but not female MS patients had significantly higher total HDL-C and small HDL-C subfraction than healthy controls. Large HDL-C negatively correlated with all measured cytokines except IL-17 in MS but not in controls. Intermediate HDL-C subfractions correlated positively with all measured cytokines except G-CSF in MS females but not in MS males or controls. Our results of higher HDL-C and mainly its small HDL-C subfraction suggest that male MS patients are at higher risk of atherosclerosis and the subtle dyslipidemia is present in early stages of the disease. The correlations between specific HDL-C subfractions and the inflammatory cytokines demonstrate mutual links between systemic inflammation and lipid metabolism in MS. ClinicalTrials.gov, Identifier: NCT 03052595 Registered on Feb 14, 2017.

中文翻译：

---

早期多发性硬化症患者的脂蛋白谱分析：慢性炎症的影响？

炎性细胞因子通过降低HDL-胆固醇（HDL-C）水平，损害其抗炎和抗氧化功能，促成脂质代谢的促动脉粥样硬化改变。因此，HDL-C的保护作用在诸如多发性硬化（MS）的慢性炎性疾病中可能受到限制。这项研究的目的是评估多发性硬化症早期阶段脂蛋白亚组分与炎症状态之间的关系。聚丙烯酰胺凝胶电泳Lipoprint©系统用于19位新诊断的MS患者和相匹配的19位健康对照者的脂蛋白谱分析。血清白介素（IL）1β，IL-2，IL-4，IL-5，IL-6，IL-7，IL-8，IL-10，IL-12（p70），IL-13，IL- 17，粒细胞集落刺激因子（G-CSF），粒细胞巨噬细胞集落刺激因子，用多重磁珠法测定干扰素-γ和TNF-α。在MS患者和对照组之间，测得的细胞因子和脂蛋白亚类的浓度相当。男性而非女性MS患者的总HDL-C和较小的HDL-C亚组明显高于健康对照组。大型HDL-C与MS中除IL-17以外的所有测得的细胞因子均呈负相关，而与对照相比则无相关性。MS女性中，HDL-C中间分数与所有测量的细胞因子（G-CSF除外）呈正相关，而男性或对照男性中则没有。我们的HDL-C升高，主要是HDL-C降低的结果表明，男性MS患者罹患动脉粥样硬化的风险更高，并且在疾病的早期阶段存在微妙的血脂异常。特定的HDL-C亚型与炎性细胞因子之间的相关性表明MS的全身性炎症与脂质代谢之间存在相互联系。ClinicalTrials.gov，标识号：NCT 03052595 2017年2月14日注册。