Herein, a novel phototheranostic nanocomplex that is self-assembled from bovine serum albumin (BSA) and indocyanine green (ICG) is developed for enhanced near-infrared (NIR) fluorescence imaging, which benefits the guidance on in vivo cancer photothermal therapy (PTT). The study confirms that the binding of ICG with the bind sits on the albumin will result in improved hydrolytic stability and high photoluminescence quantum yield (PLQY). The ICG loading ratio in the nanocomplex is optimized and confirms the loading ratio of 0.5% ICG to be the optimal content. The optimized ICG–BSA nanocomplex (ICG–BSA NC) possesses a higher PLQY of 16.8% than that of free ICG (2.7%). The high PLQY and efficient passive targeting ability of ICG–BSA NC help improve its in vivo tumor accumulation and NIR fluorescence imaging significantly. Under laser irradiation, efficient PTT with obvious tumor growth suppression on a triple negative breast tumor model can be observed in the ICG–BSA NC treated group.

中文翻译：

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合理组装的白蛋白/吲哚菁绿纳米复合物，可增强肿瘤成像，指导光热疗法

本文中，开发了一种由牛血清白蛋白（BSA）和吲哚菁绿（ICG）自组装的新型光热纳米复合物，用于增强的近红外（NIR）荧光成像，这对体内癌症光热疗法（PTT）的指导很有帮助。 。该研究证实，ICG与白蛋白上的结合基团的结合将改善水解稳定性，并提高光致发光量子产率（PLQY）。优化了纳米复合物中ICG的负载率，并确认0.5％ICG的负载率是最佳含量。优化的ICG-BSA纳米复合物（ICG-BSA NC）的PLQY高于游离ICG（2.7％）的16.8％。ICG-BSA NC的高PLQY和有效的被动靶向能力有助于显着改善其体内肿瘤积累和NIR荧光成像。在激光照射下