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Research into the intervention effect of folic acid on arsenic-induced heart abnormalities in fetal rats during the periconception period
BMC Cardiovascular Disorders ( IF 2.1 ) Pub Date : 2020-03-17 , DOI: 10.1186/s12872-020-01418-z
Lin Na , Bai Q , Zhao Xiumei , Zhuang Lingzi , He Deqin , Zhuang Xuanxuan , Guo Huanhuan , Lin Yuan , Chen Xiujuan

The incidence of CHD is the highest among birth defects and is increasing year to year. CHD seriously harms the health of infants and young children and presents a large economic burden to families and society. The pathogenesis of CHD and preventive measures are the focus of current research. Our research aimed to explore the intervention effect of folic acid on heart abnormalities resulting from sodium arsenic (NaAsO2) exposure during the periconception period. Sixty 35-day-old female SD rats were randomly divided into 5 groups with 12 rats in each group. Group A was the control group. The rats were given distilled water and ordinary chow. The rats in group B were given distilled water containing 75 mg/L NaAsO2 and ordinary chow. The rats in groups C, D, and E were given distilled water containing 75 mg/L NaAsO2 and chow containing 0.53 mg/kg, 5.3 mg/kg, and 10.6 mg/kg folic acid, respectively. The general condition of the embryos and the histopathology of the embryonic hearts were examined. The acetylation levels of histone H3K9 in heart tissues and the expression levels of Mef2C (which is related to heart development) were observed. The embryo weight and placental weight of groups B-E were significantly lower than those of group A (P < 0.05). The heart malformation rate of the fetal rats in groups B-E was significantly higher than that of the fetal rats in group A (P < 0.05). We found that the level of H3K9 acetylation in fetal rat cardiomyocytes in groups B-E was significantly higher than that in group A (P < 0.05) and that the level of H3K9 acetylation in groups C-E was lower than that in group B (P < 0.05). The mRNA level of Mef2C in fetal rat cardiomyocytes in group B-E was significantly higher than that in group A (P < 0.05), and the mRNA level of Mef2C in groups C-E was significantly lower than that in group B (P < 0.05). Supplementation with folic acid during the periconception period can interfere with the toxic effects of arsenic on the heart. The mechanism may be that lowering the acetylation levels of histone H3K9 in heart tissues leads to decreased expression levels of Mef2C, which may play a protective role in heart development in fetal rats.

中文翻译:

叶酸对孕期砷致胎鼠心脏异常的干预作用研究

冠心病的发生率在先天缺陷中最高,并且逐年增加。冠心病严重危害婴幼儿的健康,给家庭和社会带来沉重的经济负担。冠心病的发病机制和预防措施是当前研究的重点。我们的研究旨在探讨叶酸对受孕期砷酸钠(NaAsO2)暴露引起的心脏异常的干预作用。60只35日龄雌性SD大鼠随机分为5组,每组12只。A组为对照组。给大鼠蒸馏水和普通的食物。B组大鼠给予蒸馏水,其中含有75 mg / L NaAsO2和普通食物。C,D和E组的大鼠分别接受75 mg / L NaAsO2的蒸馏水和0.53 mg / kg 5的食物。叶酸分别为3 mg / kg和10.6 mg / kg。检查了胚胎的一般状况和胚胎心脏的组织病理学。观察到心脏组织中组蛋白H3K9的乙酰化水平和Mef2C的表达水平(与心脏发育有关)。BE组的胚胎重量和胎盘重量均显着低于A组(P <0.05)。BE组胎鼠的心脏畸形率明显高于A组胎鼠(P <0.05)。我们发现,BE组的胎鼠心肌细胞中的H3K9乙酰化水平明显高于A组(P <0.05),CE组的H3K9乙酰化水平低于B组(P <0.05) 。BE组胎鼠心肌细胞Mef2C的mRNA水平明显高于A组(P <0.05),CE组Mef2C的mRNA水平显着低于B组(P <0.05)。在围孕期补充叶酸会干扰砷对心脏的毒性作用。其机制可能是降低心脏组织中组蛋白H3K9的乙酰化水平会导致Mef2C表达水平降低,这可能在胎鼠心脏发育中起保护作用。
更新日期:2020-03-19
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