The cerebellum improves motor performance by adjusting motor gain appropriately. As de novo protein synthesis is essential for the formation and retention of memories, we hypothesized that motor learning in the opposite direction would induce a distinct pattern of protein expression in the cerebellum. We conducted quantitative proteomic profiling to compare the level of protein expression in the cerebellum at 1 and 24 h after training from mice that underwent different paradigms of cerebellum-dependent oculomotor learning from specific directional changes in motor gain. We quantified a total of 43 proteins that were significantly regulated in each of the three learning paradigms in the cerebellum at 1 and 24 h after learning. In addition, functional enrichment analysis identified protein groups that were differentially enriched or depleted in the cerebellum at 24 h after the three oculomotor learnings, suggesting that distinct biological pathways may be engaged in the formation of three oculomotor memories. Weighted correlation network analysis discovered groups of proteins significantly correlated with oculomotor memory. Finally, four proteins (Snca, Sncb, Cttn, and Stmn1) from the protein group correlated with the learning amount after oculomotor training were validated by Western blot. This study provides a comprehensive and unbiased list of proteins related to three cerebellum-dependent motor learning paradigms, suggesting the distinct nature of protein expression in the cerebellum for each learning paradigm. The proteomics data have been deposited to the ProteomeXchange Consortium with identifiers .

中文翻译：

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定量蛋白质组学揭示了不同的依赖于小脑的学习范例的分子特征。

小脑通过适当调节电动机增益来改善电动机性能。由于从头进行蛋白质合成对于记忆的形成和保持至关重要，因此我们假设在相反的方向进行运动学习会诱导小脑中蛋白质表达的独特模式。我们进行了定量蛋白质组分析，以比较接受运动模式特定变化的小脑依赖动眼学习的小鼠训练后1和24 h小脑中蛋白质表达的水平。我们在学习后的1和24小时内，对小脑的三种学习范式中的每种显着调节的43种蛋白质进行了定量分析。此外，功能富集分析确定了三种动眼运动学习后24小时内小脑中差异富集或耗尽的蛋白质组，表明不同的生物学途径可能参与了三种动眼运动记忆的形成。加权相关网络分析发现与动眼记忆显着相关的蛋白质组。最后，通过蛋白质印迹法验证了该蛋白组中的四种蛋白（Snca，Sncb，Cttn和Stmn1）与动眼神经训练后的学习量相关。这项研究提供了与三种依赖于小脑的运动学习范例相关的蛋白质的全面且公正的清单，表明每种学习范例在小脑中蛋白质表达的独特性质。。