Surfaces with specific topography and chemical composition are quite useful in many applications ranging from functional interfaces to cell incubation scaffolds. Although these surfaces can be easily fabricated by combining topography-construction methods and surface-functionalization strategies, their properties are often static after fabrication or merely switchable between “on” and “off” states. Developing surfaces that can be on-demand regulated are quite important for the generation of smart surfaces for future applications. In this paper, we present a reconfigurable surface with adjustable topography and chemical functionality utilizing the photodynamic feature of the disulfide bond. Structured surfaces, composed of disulfide-cross-linked polymer networks, were prepared by using disulfide-containing methacrylate as the monomer. We show that the topography and chemical functionality of the surface can be on-demand regulated after its fabrication, with 254 and 365 nm UV light, respectively, allowing to “define” the physicochemical properties of the surface using light before the usage. We also demonstrate the application of such surface as a user-designable cell scaffold, that different cell scaffolds can be generated from one original surface with a simple exposure process, to define the desired bioactivity onto every point of the surface and therefore exactly control cell behaviors on the scaffold.

中文翻译：

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用于用户可设计细胞支架的具有光可定义物理化学特性的可重构表面

具有特定形貌和化学成分的表面在从功能界面到细胞培养支架的许多应用中都非常有用。尽管这些表面可以通过结合形貌构造方法和表面功能化策略轻松制造，但它们的特性在制造后通常是静态的，或者仅可在“开”和“关”状态之间切换。开发可按需调节的表面对于为未来应用生成智能表面非常重要。在本文中，我们利用二硫键的光动力学特性，提出了一种具有可调节形貌和化学功能的可重构表面。以含二硫化物的甲基丙烯酸酯为单体，制备了由二硫化物交联的聚合物网络组成的结构化表面。我们表明，表面的形貌和化学功能可以在其制造后按需调节，分别使用 254 和 365 nm 紫外光，允许在使用前使用光“定义”表面的物理化学性质。我们还展示了这种表面作为用户可设计细胞支架的应用，可以通过简单的暴露过程从一个原始表面生成不同的细胞支架，以在表面的每个点上定义所需的生物活性，从而精确控制细胞行为在脚手架上。