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Gene network transitions in embryos depend upon interactions between a pioneer transcription factor and core histones.
Nature Genetics ( IF 30.8 ) Pub Date : 2020-03-16 , DOI: 10.1038/s41588-020-0591-8
Makiko Iwafuchi 1, 2 , Isabel Cuesta 3 , Greg Donahue 1 , Naomi Takenaka 1 , Anna B Osipovich 4 , Mark A Magnuson 4 , Heinrich Roder 5 , Steven H Seeholzer 6 , Pilar Santisteban 3, 7 , Kenneth S Zaret 1
Affiliation  

Gene network transitions in embryos and other fate-changing contexts involve combinations of transcription factors. A subset of fate-changing transcription factors act as pioneers; they scan and target nucleosomal DNA and initiate cooperative events that can open the local chromatin. However, a gap has remained in understanding how molecular interactions with the nucleosome contribute to the chromatin-opening phenomenon. Here we identified a short α-helical region, conserved among FOXA pioneer factors, that interacts with core histones and contributes to chromatin opening in vitro. The same domain is involved in chromatin opening in early mouse embryos for normal development. Thus, local opening of chromatin by interactions between pioneer factors and core histones promotes genetic programming.



中文翻译:

胚胎中的基因网络转换取决于先驱转录因子和核心组蛋白之间的相互作用。

胚胎和其他改变命运的环境中的基因网络转换涉及转录因子的组合。一部分改变命运的转录因子充当先驱;他们扫描并靶向核小体 DNA,并启动可以打开局部染色质的合作事件。然而,在理解分子与核小体的相互作用如何促成染色质开放现象方面仍然存在差距。在这里,我们发现了一个在 FOXA 先驱因子中保守的短 α-螺旋区域,它与核心组蛋白相互作用并有助于体外染色质开放。相同的结构域参与早期小鼠胚胎正常发育的染色质开放。因此,通过先驱因子和核心组蛋白之间的相互作用,染色质的局部开放促进了遗传编程。

更新日期:2020-04-24
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