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Genetically encoded protein sulfation in mammalian cells.
Nature Chemical Biology ( IF 14.8 ) Pub Date : 2020-03-16 , DOI: 10.1038/s41589-020-0493-1
James S Italia 1 , Jennifer C Peeler 1 , Christen M Hillenbrand 1 , Christopher Latour 1 , Eranthie Weerapana 1 , Abhishek Chatterjee 1
Affiliation  

Tyrosine sulfation is an important post-translational modification found in higher eukaryotes. Here we report an engineered tyrosyl-tRNA synthetase/tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in Escherichia coli and mammalian cells. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites, which was demonstrated by expressing human heparin cofactor II in mammalian cells in different states of sulfation.



中文翻译:

哺乳动物细胞中遗传编码的蛋白质硫酸化。

酪氨酸硫酸化是在高等真核生物中发现的重要的翻译后修饰。在这里,我们报道了一种工程化的酪氨酰-tRNA合成酶/ tRNA对,它在大肠杆菌和哺乳动物细胞中响应UAG密码子而共翻译结合了O-磺基酪氨酸。该平台能够重组表达在选定位点被均匀硫酸化的真核蛋白,这通过在不同硫酸化状态的哺乳动物细胞中表达人肝素辅因子II来证明。

更新日期:2020-04-24
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