Human leukocyte antigen (HLA) class I molecules present antigenic peptides to cytotoxic T cells, causing lysis of malignant cells. Transplantation biology studies have implicated HLA class I molecules in cell migration, but there has been little evidence presented that they influence cancer cell migration, a contributing factor in metastasis. In this study, we examined the effect of HLA-B on pancreatic cancer cell migration. HLA-B siRNA transfection increased the migration of the S2-013 pancreatic cancer cells but, in contrast, reduced migration of the PANC-1 and MIA PaCa-2 pancreatic cancer cell lines. Integrin molecules have previously been implicated in the upregulation of pancreatic cancer cell migration, and knockdown of HLA-B in S2-013 cells heightened the expression of integrin beta 1 (ITGB1), but in the PANC-1 and MIA PaCa-2 cells HLA-B knockdown diminished ITGB1 expression. A transmembrane sequence in an S2-013 HLA-B heavy chain matches a corresponding sequence in HLA-B in the BxPC-3 pancreatic cancer cell line, and knockdown of BxPC-3 HLA-B mimics the effect of S2-013 HLA-B knockdown on migration. In total, our findings indicate that HLA-B influences the expression of ITGB1 in pancreatic cancer cells, with concurrent distinctions in transmembrane sequences and effects on the migration of the cells.

中文翻译：

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HLA-B 影响整合素 beta-1 表达和胰腺癌细胞迁移。

人类白细胞抗原 (HLA) I 类分子将抗原肽呈递给细胞毒性 T 细胞，导致恶性细胞裂解。移植生物学研究表明 HLA I 类分子与细胞迁移有关，但几乎没有证据表明它们会影响癌细胞迁移，这是转移的一个促成因素。在这项研究中，我们检查了 HLA-B 对胰腺癌细胞迁移的影响。HLA-B siRNA 转染增加了 S2-013 胰腺癌细胞的迁移，但相反，减少了 PANC-1 和 MIA PaCa-2 胰腺癌细胞系的迁移。整合素分子先前被认为与胰腺癌细胞迁移的上调有关，S2-013细胞中HLA-B的敲低会提高整合素β1（ITGB1）的表达，但在PANC-1和MIA PaCa-2细胞中HLA的表达升高-B 敲低减少了 ITGB1 表达。S2-013 HLA-B 重链中的跨膜序列与 BxPC-3 胰腺癌细胞系中 HLA-B 中的相应序列相匹配，并且 BxPC-3 HLA-B 的敲低模拟了 S2-013 HLA-B 的作用抑制移民。总的来说，我们的研究结果表明 HLA-B 影响胰腺癌细胞中 ITGB1 的表达，同时跨膜序列存在差异，并对细胞迁移产生影响。