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Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice
Lipids in Health and Disease ( IF 4.5 ) Pub Date : 2020-03-16 , DOI: 10.1186/s12944-020-01231-9
Maria Shabbir , Tayyaba Afsar , Suhail Razak , Ali Almajwal , Muhammad Rashid Khan

Myrin®-p Forte is an anti-tuberclosis agent that can cause hepatic injuries in clinical settings. Maytenus royleanus (Celastraceae) is a medicinal plant, possesses antioxidant and anticancer activities. The hepatoprotective effect of the methanol extract of Maytenus royleanus leaves (MEM) against Myrin®-p Forte induced hepatotoxicity in mice was investigated. Mice were randomly parted into six groups (n = 6). Fixed-dose combination of Myrin®-p Forte (13.5 mg/kg Rifampicin, 6.75 mg/kg Isoniazid, 36.0 mg/kg Pyrazinamide and 24.8 mg/kg Ethambutol; RIPE] was administered for 15 days to induce liver injury. In treatment groups MEM (200 mg/kg and 400 mg/kg doses) and Vitamin B6 (180mg/kg) were administered prior to RIPE. Control group received 2% DMSO. Serum liver function tests, DNA damage, tissue antioxidant enzymes and histopathological alterations were studied. HPLC analysis was performed to determine the chemical composition using standard compounds. The quercitin, gallic acid, luteolin, viteixin, apigenin, kaempherol, hyperoside and myricetin contents of all samples were determined by reverse-phase HPLC. Quercetin (0.217 mg/g dry weight) and luteolin (0.141 mg/g dry weight) were the major flavonoids identified in MEM. Myrin®-p Forte markedly (p < 0.05) deteriorated lipid profile and upregulated the concentration of LDH, AST, ALP, ALT and γ-GT in serum along with DNA fragmentation (37.13 ± 0.47%) and histopathological injuries in hepatic tissues of mice compared with the control group. Myrin®-p Forte increased (p < 0.001) lipid peroxidation and H2O2 while decreased (p < 0.001) the activity level of CAT, SOD, POD, GPx, GST, GSR, γ-GT and GSH. Co-administration of MEM (200 mg/kg; 400 mg/kg) or the vitamin B6 (180 mg/kg) to Myrin®-p Forte administered mice significantly ameliorated LDL, cholesterol, HDL and triglyceride content. Furthermore, MEM dose dependently corrected serum liver function tests, decrease % DNA fragmentation (17.82 ± 0.35 and 7.21 ± 0.32 respectively), DNA damage. MEM treated protect RIPE induced oxidative damage by enhancing antioxidants to oxidants balance. Histological examination comprehends biochemical findings. The antioxidant effects of MEM exerted the hepatoprotective potential against the Myrin®-p Forte induced hepatotoxicity in mice.

中文翻译:

红叶美登木叶提取物对小鼠抗结核药性肝损伤的植物化学分析及保肝作用的评价

Myrin®-pForte是一种抗结核药,在临床环境中会引起肝损伤。柳叶Maytenus royleanus(Celastraceae)是药用植物,具有抗氧化和抗癌活性。研究了美登红叶(Maytenus royleanus)叶片(MEM)的甲醇提取物对Myrin®-pForte诱导的小鼠肝毒性的肝保护作用。将小鼠随机分为六组(n = 6)。固定剂量的Myrin®-pForte(13.5 mg / kg利福平,6.75 mg / kg异烟肼,36.0 mg / kg吡嗪酰胺和24.8 mg / kg乙胺丁醇; RIPE)联合给药15天,以引起肝损伤。在进行RIPE之前先服用MEM(200 mg / kg和400 mg / kg剂量)和维生素B6(180mg / kg),对照组接受2%DMS​​O,进行血清肝功能检查,DNA损伤,研究了组织抗氧化酶和组织病理学改变。使用标准化合物进行HPLC分析以确定化学组成。通过反相HPLC测定所有样品中的槲皮素,没食子酸,木犀草素,viteixin,芹菜素,kaempherol,hyperoside和杨梅素含量。槲皮素(0.217 mg / g干重)和木犀草素(0.141 mg / g干重)是MEM中鉴定出的主要类黄酮。Myrin®-pForte显着(p <0.05)降低了脂质谱并上调了血清LDH,AST,ALP,ALT和γ-GT的浓度以及小鼠肝组织的DNA片段化(37.13±0.47%)和组织病理学损伤与对照组相比。Myrin®-pForte增加(p <0.001)脂质过氧化和过氧化氢,同时降低(p <0.001)CAT,SOD,POD,GPx,GST,GSR,γ-GT和GSH。将MEM(200 mg / kg; 400 mg / kg)或维生素B6(180 mg / kg)共同施用给Myrin®-pForte施用的小鼠,可显着改善LDL,胆固醇,HDL和甘油三酸酯的含量。此外,MEM剂量依赖性地校正了血清肝功能测试,降低了%DNA片段化(分别为17.82±0.35和7.21±0.32),DNA损伤。MEM处理可通过增强抗氧化剂与氧化剂的平衡来保护RIPE诱导的氧化损伤。组织学检查包括生化检查结果。MEM的抗氧化作用具有抗Myrin®-pForte诱导的小鼠肝毒性的肝保护潜力。HDL和甘油三酸酯含量。此外,MEM剂量依赖性地校正了血清肝功能测试,降低了%DNA片段化(分别为17.82±0.35和7.21±0.32),DNA损伤。MEM处理可通过增强抗氧化剂与氧化剂的平衡来保护RIPE诱导的氧化损伤。组织学检查包括生化检查结果。MEM的抗氧化作用具有抗Myrin®-pForte诱导的小鼠肝毒性的肝保护潜力。HDL和甘油三酸酯含量。此外,MEM剂量依赖性地校正了血清肝功能测试,降低了%DNA片段化(分别为17.82±0.35和7.21±0.32),DNA损伤。MEM处理可通过增强抗氧化剂与氧化剂的平衡来保护RIPE诱导的氧化损伤。组织学检查包括生化检查结果。MEM的抗氧化作用具有抗Myrin®-pForte诱导的小鼠肝毒性的肝保护潜力。
更新日期:2020-04-22
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