Trehalose plays important roles in plant growth and stress responses and is synthesized from trehalose‐6‐phosphate by trehalose‐6‐phosphate phosphatase (TPP). Here, we show that trehalose and abscisic acid (ABA) have synergistic effects on root growth and stomatal closure. The Arabidopsis thaliana genome contains ten genes encoding TPPs and the expression level of one, TPPE, and trehalose contents increased in response to ABA. In the presence of ABA, the ABA‐responsive transcription factor ABA RESPONSE ELEMENT BINDING FACTOR2 (ABF2) directly binds to the TPPE promoter to activate its expression. Genetic analysis revealed that TPPE acts downstream of ABF2, which is supported by the findings that TPPE expression and trehalose content are reduced in the abf2 mutant and that a mutation in TPPE abolished the ABA‐sensitive root elongation phenotype of 35S:ABF2 plants. Reactive oxygen species (ROS) accumulation in response to ABA failed to occur in tppe mutant plants, suggesting that TPPE is involved in ABA‐controlled root elongation and stomatal movement by inducing ROS accumulation. This study uncovers a new branch of the ABA signaling pathway and provides a molecular basis for the role of trehalose in plant responses to abiotic stress.

中文翻译：

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海藻糖6-磷酸磷酸酶E调节拟南芥中ABA控制的根系生长和气孔运动

海藻糖在植物生长和胁迫响应中起着重要作用，由海藻糖6-磷酸与海藻糖-6-磷酸磷酸酶（TPP）合成而成。在这里，我们显示海藻糖和脱落酸（ABA）对根生长和气孔闭合具有协同作用。在拟南芥基因组中包含编码和的TPP中的一个，所述表达水平10个基因TPPE和海藻糖内容响应于ABA增加。在存在ABA的情况下，ABA响应转录因子ABA响应元素结合因子2（ABF2）直接与TPPE启动子结合以激活其表达。遗传分析表明TPPE在ABF2的下游起作用，这一发现得到了以下支持：TPPE表达和海藻糖含量在降低ABF2突变体和在一个突变TPPE废除的ABA敏感根伸长的表型ABF2：35S植物。在tppe突变植物中未能响应ABA发生活性氧（ROS）积累，这表明TPPE通过诱导ROS积累参与了ABA控制的根伸长和气孔运动。这项研究发现了ABA信号传导途径的一个新分支，并为海藻糖在植物对非生物胁迫的反应中的作用提供了分子基础。