High‐pressure (HP) pre‐treatments were applied on European hake (Merluccius merluccius) followed by a frozen accelerated experiment (−10 °C). A central composite design ranging pressure levels (150–450 MPa) and frozen storage time (0–150 days) was used, being evaluated the enzymatic activities and muscle proteins. Acid phosphatase and calpain activities decreased after 150 days of frozen storage (58%/56% and 38%/56% for non‐/HP‐treated samples, respectively). Cathepsin B showed higher reductions (98%) for longer storage times. Furthermore, HP and frozen storage did not affect significantly cathepsin D activity, only slightly decreasing at 169 MPa. Furthermore, HP seemed to not affect myofibrillar proteins, while sarcoplasmic proteins were clearly affected by HP and frozen storage time, resulting in a reduction of about 53% or 23% for 431 or 450 MPa, respectively. Thus, HP could be used to lowering the deleterious effect of proteases on frozen European hake.

中文翻译：

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高压预处理的冷冻无须鳕（Merluccius merluccius）的酶活性以及肌浆蛋白和肌原纤维蛋白的变化

对欧洲无须鳕（Merluccius merluccius）进行了高压（HP）预处理），然后进行冻结的加速实验（-10°C）。使用了压力范围（150–450 MPa）和冷冻储存时间（0–150天）范围内的中央复合材料设计，评估了酶活性和肌肉蛋白。冷冻保存150天后，酸性磷酸酶和钙蛋白酶的活性下降（未经/经过HP处理的样品分别为58％/ 56％和38％/ 56％）。组织蛋白酶B表现出更高的减少率（98％），可延长储存时间。此外，HP和冷冻储存不会显着影响组织蛋白酶D的活性，只是在169 MPa时略有下降。此外，HP似乎不影响肌原纤维蛋白，而肌浆蛋白明显受到HP和冷冻保存时间的影响，导致431或450 MPa分别降低了约53％或23％。从而，