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Biomaterials and biosensors in intestinal organoid culture, a progress review.
Journal of Biomedical Materials Research Part A ( IF 4.9 ) Pub Date : 2020-03-20 , DOI: 10.1002/jbm.a.36921
Jinjian Huang 1, 2 , Yungang Jiang 1, 2 , Yanhan Ren 3 , Ye Liu 1, 2 , Xiuwen Wu 2 , Zongan Li 4 , Jianan Ren 1, 2
Affiliation  

As an emerging technology, intestinal organoids are promising new tools for basic and translational research in gastroenterology. Currently, culture of intestinal organoids relies mostly on a type of tumor‐derived scaffolds, namely Matrigel, which may pose tumorigenic risks to organoid implantation. Apart from the traditional detection methods, such as tissue slicing and fluorescence staining, the monitoring of intestinal organoids requires real‐time biosensors that can adapt to their three‐dimensional dynamic growth patterns. In this review, we summarized the recent advances in developing definite hydrogel scaffolds for intestinal organoid culture and identified key parameters for scaffold design. In addition, classified by different substrate compositions like pH, electrolytes, and functional proteins, we concluded the existing live‐imaging biosensors and elucidated their underlying mechanisms. We hope this review enhances the understanding of intestinal organoid culture and provides more practical approaches to investigate them.

中文翻译:

肠道类器官培养中的生物材料和生物传感器,进展综述。

作为一项新兴技术,肠道类器官是胃肠病学基础和转化研究的有前途的新工具。目前,肠道类器官的培养主要依赖于一种肿瘤来源的支架,即 Matrigel,它可能对类器官植入造成致瘤风险。除了传统的检测方法,如组织切片和荧光染色,肠道类器官的监测需要能够适应其三维动态生长模式的实时生物传感器。在这篇综述中,我们总结了开发用于肠道类器官培养的特定水凝胶支架的最新进展,并确定了支架设计的关键参数。此外,根据不同的底物组成,如 pH、电解质和功能性蛋白质,我们总结了现有的实时成像生物传感器并阐明了它们的潜在机制。我们希望这篇综述能增进对肠道类器官培养的理解,并提供更实用的方法来研究它们。
更新日期:2020-03-20
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