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A 3-Dimensional Bioprinted Scaffold With Human Umbilical Cord Blood-Mesenchymal Stem Cells Improves Regeneration of Chronic Full-Thickness Rotator Cuff Tear in a Rabbit Model.
The American Journal of Sports Medicine ( IF 4.8 ) Pub Date : 2020-03-13 , DOI: 10.1177/0363546520904022
Dong Rak Kwon 1 , Seungman Jung 2 , Jinah Jang 2, 3, 4 , Gi-Young Park 1 , Yong Suk Moon 5 , Sang Chul Lee 6
Affiliation  

Background:

Chronic full-thickness rotator cuff tears (FTRCTs) represent a major clinical concern because they show highly compromised healing capacity.

Purpose:

To evaluate the efficacy of using a 3-dimensional (3D) bioprinted scaffold with human umbilical cord blood (hUCB)–mesenchymal stem cells (MSCs) for regeneration of chronic FTRCTs in a rabbit model.

Study Design:

Controlled laboratory study.

Methods:

A total of 32 rabbits were randomly assigned to 4 treatment groups (n = 8 per group) at 6 weeks after a 5-mm FTRCT was created on the supraspinatus tendon. Group 1 (G1-SAL) was transplanted with normal saline. Group 2 (G2-MSC) was transplanted with hUCB-MSCs (0.2 mL, 1 × 106) into FTRCTs. Group 3 (G3-3D) was transplanted with a 3D bioprinted construct without MSCs, and group 4 (G4-3D+MSC) was transplanted with a 3D bioprinted construct containing hUCB-MSCs (0.2 mL, 1 × 106 cells) into FTRCTs. All 32 rabbits were euthanized at 4 weeks after treatment. Examination of gross morphologic changes and histologic results was performed on all rabbits after sacrifice. Motion analysis was also performed before and after treatment.

Results:

In G4-3D+MSC, newly regenerated collagen type 1 fibers, walking distance, fast walking time, and mean walking speed were greater than those in G2-MSC based on histochemical and motion analyses. In addition, when compared with G3-3D, G4-3D+MSC showed more prominent regenerated tendon fibers and better parameters of motion analysis. However, there was no significant difference in gross tear size among G2-MSC, G3-3D, and G4-3D+MSC, although these groups showed significant decreases in tear size as compared with the control group (G1-SAL).

Conclusion:

Findings of this study show that a tissue engineering strategy based on a 3D bioprinted scaffold filled with hUCB-MSCs can improve the microenvironment for regenerative processes of FTRCT without any surgical repair.

Clinical Relevance:

In the case of rotator cuff tear, the cell loss of the external MSCs can be increased by exposure to synovial fluid. Therefore, a 3D bioprinted scaffold in combination with MSCs without surgical repair may be effective in increasing cell retention in FTRCT.



中文翻译:

具有人脐带血间充质干细胞的三维生物打印支架可改善兔子模型中慢性全厚度肩袖撕裂的再生。

背景:

慢性全层肩袖撕裂症(FTRCT)代表了主要的临床问题,因为它们显示出严重损害的愈合能力。

目的:

为了评估将三维(3D)生物打印支架与人脐带血(hUCB)-间充质干细胞(MSC)结合用于兔模型中慢性FTTRT的疗效。

学习规划:

对照实验室研究。

方法:

在棘上肌腱上建立5mm FTRCT后,在6周时将总共32只兔子随机分为4个治疗组(每组n = 8)。第1组(G1-SAL)移植有生理盐水。将第2组(G2-MSC)与hUCB-MSC(0.2 mL,1×10 6)移植到FTRCT中。将第3组(G3-3D)移植有没有MSC的3D生物打印构建体,将第4组(G4-3D + MSC)移植有包含hUCB-MSC(0.2 mL,1×10 6个细胞)的3D生物打印构建体。 。在治疗后4周对所有32只兔子实施安乐死。处死后,对所有兔子进行大体形态变化和组织学检查。在治疗前后也进行运动分析。

结果:

根据组织化学和运动分析,在G4-3D + MSC中,新再生的1型胶原纤维,步行距离,快速步行时间和平均步行速度大于G2-MSC。此外,与G3-3D相比,G4-3D + MSC表现出更突出的再生肌腱纤维和更好的运动分析参数。但是,尽管G2-MSC,G3-3D和G4-3D + MSC的总眼泪大小没有显着差异,尽管与对照组(G1-SAL)相比,这些组的眼泪大小明显减少。

结论:

这项研究的结果表明,基于3h生物打印支架填充hUCB-MSC的组织工程策略可以改善FTRCT再生过程的微环境,而无需任何手术修复。

临床相关性:

在肩袖撕裂的情况下,通过暴露于滑液可以增加外部MSC的细胞损失。因此,无需手术修复的3D生物打印支架与MSC的组合可能有效增加FTRCT中的细胞保留。

更新日期:2020-03-16
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