Antibiotics from the amphenicol family are widely used to treat farm animals; however, the regulation of their residues in livestock production varies significantly and depends on the type of animal and the type of particular amphenicol. In this regard, different requirements are imposed on immunoanalytical systems for their identification. In the present study, direct and indirect ELISA formats were developed for simultaneous determination of florfenicol (FF), its main metabolite florfenicol amine (FFA), and thiamphenicol (TAP) using antibodies against FFA conjugated to succinylated BSA. Among a panel of heterologous conjugates, a glutaraldehyde linked FFA-coating antigen and tracer were the only ones which provided equal recognition of FFA, FF, and TAP. Owing to equal 100% cross-reactivity, these analytes could be quantified in chicken muscle at their MRL level using a single universal standard curve. Recovery of amphenicols was in the range 77.2–98.8% with variation no more than 9.6%. The prohibited drug chloramphenicol was undetectable (<0.1%) and had no influence on the assay developed for quantification of approved amphenicols.

中文翻译：

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三只一的价格！使用通用标准曲线对食品中的三种安非他命进行免疫检测

氨苯酚家族的抗生素被广泛用于治疗牲畜。然而，它们在畜牧业生产中的残留物调节差异很大，并且取决于动物的类型和特定的苯甲酚的类型。在这方面，对免疫分析系统的鉴定提出了不同的要求。在本研究中，开发了直接和间接ELISA格式，可使用与琥珀酰化BSA偶联的FFA抗体同时测定氟苯尼考（FF），其主要代谢物氟苯尼考胺（FFA）和甲砜霉素（TAP）。在一组异源结合物中，只有戊二醛连接的FFA-包被抗原和示踪剂可以提供对FFA，FF和TAP的相同识别。由于交叉反应的百分率相等，这些分析物可以使用一条通用标准曲线以其最大残留限量（MRL）在鸡肉中定量。两性霉素的回收率在77.2–98.8％范围内，变化不超过9.6％。禁止使用的药物氯霉素不可检出（<0.1％），对开发用于批准的安非他酮定量的测定法没有影响。