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Effect of sodium and calcium on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Lentinus edodes
AMB Express ( IF 3.7 ) Pub Date : 2020-03-14 , DOI: 10.1186/s13568-020-00985-w
Bilal Adil , Quanju Xiang , Maolan He , Yuetong Wu , Muhammad Ahsan Asghar , Muhammad Arshad , Peng Qin , Yunfu Gu , Xiumei Yu , Ke Zhao , Xiaoping Zhang , Menggen Ma , Qiang Chen , Xiaoqiong Chen , Yanhong Yan

Abstract

Lentinan is a Lentinus edodes secondary metabolite that can regulate human immune function, but yields are low. Here, the effects of Ca2+ and Na+ on L. edodes lentinan content were investigated. Metal ion concentrations and induction times were optimized according to mycelial biomass, and intracellular polysaccharide (IPS), extracellular polysaccharide (EPS), and total polysaccharide (TPS) content. The activities and gene expression of phospho-glucose isomerase (PGI), phosphoglucomutase (PGM), and UDP-glcpyrophosphorylase (UGP) were also measured. Ca2+ and Na+ concentration and induction time affected biomass, IPS, and EPS concentrations. Na+ increased EPS, IPS and TPS, while Ca2+ increased biomass, IPS, and TPS. During fermentation, mycelial biomass varied greatly under Ca2+ induction, while IPS, EPS and TPS varied greatly under Na+ induction. PGM and UGP activities increased in the presence of Na+, while PGI increased with Ca2+. Compared to control samples, pgi and pgm expression under Na+ was greater at days 45 and 60, respectively, while under Ca2+, ugp expression was greater at day 45. IPS content correlated significantly with enzyme activity, while EPS correlated with PGM activity. Our data contributes to better understanding how Na+ and Ca2+ affect mycelial growth and secondary metabolite production, and of polysaccharide biosynthesis mechanisms of L. edodes.



中文翻译:

钠和钙对香菇多糖合成及多糖合成酶活性的影响。

摘要

香菇多糖是香菇,可以调节人体免疫功能次级代谢产物,但是产量低。在此,研究了Ca 2+和Na +香菇Lentinan含量的影响。根据菌丝体生物量,细胞内多糖(IPS),细胞外多糖(EPS)和总多糖(TPS)含量优化了金属离子浓度和诱导时间。还测量了磷酸葡萄糖异构酶(PGI),磷酸葡萄糖变位酶(PGM)和UDP-葡糖焦磷酸化酶(UGP)的活性和基因表达。Ca 2+和Na +浓度以及诱导时间会影响生物量,IPS和EPS浓度。钠+增加EPS,IPS和TPS,而Ca 2+增加生物量,IPS和TPS。在发酵过程中,菌丝生物量在Ca 2+诱导下变化很大,而IPS,EPS和TPS在Na +诱导下变化很大。在Na +存在下PGM和UGP活性增加,而在Ca 2+下PGI增加。与对照样品相比,Na +下的pgipgm表达分别在第45天和60天更高,而在Ca 2+下ugp在第45天表达水平更高。IPS含量与酶活性显着相关,而EPS与PGM活性相关。我们的数据有助于更好地理解Na +和Ca 2+如何影响菌丝体生长和次生代谢产物以及香菇的多糖生物合成机制。

更新日期:2020-03-16
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