Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.

中文翻译：

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用于检测成年开花植物中木瓜蜜eira病毒复合体的多重RT-PCR方法。

木瓜黏性疾病（PSD）可以破坏果园，最早归因于木瓜蜜eira病毒（PMeV）。但是，发现与PSD植物相关的木瓜蜜virus病毒2（PMeV2）要求检测这种病毒复合物。我们开发了一种多重RT-PCR（mPCR）技术，该技术能够在一次测定中从开花前的植物样品中检测两种病毒，这是早期诊断PSD的有用工具。我们还使用PMeV和PMeV2的不对称质粒稀释液确定了检出限（LOD），这表明一种病毒的较高滴度可防止另一种病毒的检测。因此，该技术是在单个反应中检测PMeV和PMeV2的替代方法。