Abstract In this research we are proposing a combination of scanning electrochemical microscopy (SECM) and fast Fourier transform – electrochemical impedance spectroscopy (FFT-EIS) as a new tool, which we entitled ‘fast Fourier transform – scanning electrochemical impedance microscopy (FFT-SEIM)’ suitable for the evaluation and treatment of highly complicated electrochemical systems, in which the electrical signal is under limitations of very small surface area of the electrode and Faradaic process is controlled by slow diffusion of species in the solution. The identification and in situ treatment of unhealthy tissues at cellular level is futuristic direction of the biomedical research, which requires completely new tools and advanced techniques. In this manuscript we show that the combination of SECM and FFT-EIS systems can operate well in the absence of the additional redox probes, which are harmful for living cells and tissues. We are also addressing how some challenging electrochemical problems, which are arising during the application of FFT-SEIM in the absence of redox probe, can be solved. Particularly, in this research we have demonstrated how the quality of FFT-SEIM signal can be increased by the adjustment of FFT-SEIM parameters. Particularly, the reduction of preconditioning duration before the start of the FFT-EIS measurement and very short duration of FFT-EIS measurement, which takes just a little bit longer time than that required to register single period of sin-wave at lowest frequency of registered EIS spectra, significantly increased the quality of gathered EIS spectra.

中文翻译：

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快速傅里叶变换的应用-扫描电化学阻抗显微镜（FFT-SEIM）

摘要 在这项研究中，我们提出将扫描电化学显微镜 (SECM) 和快速傅里叶变换-电化学阻抗谱 (FFT-EIS) 结合作为一种新工具，我们将其命名为“快速傅里叶变换-扫描电化学阻抗显微镜 (FFT-SEIM)”。 )'适用于高度复杂的电化学系统的评估和处理，其中电信号受到电极表面积非常小的限制，法拉第过程由溶液中物质的缓慢扩散控制。在细胞水平上对不健康组织的识别和原位治疗是生物医学研究的未来方向，需要全新的工具和先进的技术。在这份手稿中，我们展示了 SECM 和 FFT-EIS 系统的组合在没有额外的氧化还原探针的情况下可以很好地运行，这些探针对活细胞和组织有害。我们还在解决如何解决一些具有挑战性的电化学问题，这些问题是在没有氧化还原探针的情况下应用 FFT-SEIM 期间出现的。特别是，在这项研究中，我们展示了如何通过调整 FFT-SEIM 参数来提高 FFT-SEIM 信号的质量。特别是在 FFT-EIS 测量开始前的预处理时间缩短，FFT-EIS 测量持续时间非常短，仅比在最低频率记录单周期正弦波所需的时间长一点点。 EIS 光谱，显着提高了收集的 EIS 光谱的质量。